Genome-resolved metaproteogenomic and nanosolid characterization of an inactive vent chimney densely colonized by enigmatic DPANN archaea.

Autor:	Takamiya H; Department of Earth and Planetary Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan., Kouduka M; Department of Earth and Planetary Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan., Kato S; Japan Collection of Microorganisms (JCM), RIKEN BioResource Research Center, Tsukuba, Ibaraki, Japan.; Submarine Resources Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15, Natsushima-cho, Yokosuka-city, Kanagawa 237-0061, Japan., Suga H; Spectroscopy Division, Japan Synchrotron Radiation Research Institute, Sayo-gun, Hyogo, Japan.; Graduate School of Advanced Science and Engineering, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima, Japan., Oura M; Soft X-ray Spectroscopy Instrumentation Team, RIKEN SPring-8 Center, Sayo-gun, Hyogo, Japan., Yokoyama T; Graduate School of Advanced Science and Engineering, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima, Japan., Suzuki M; Department of Applied Biological Chemistry, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan., Mori M; Institute for Advanced Biosciences, Keio University, Nipponkoku, Daihoji, Tsuruoka, Yamagata, Japan., Kanai A; Institute for Advanced Biosciences, Keio University, Nipponkoku, Daihoji, Tsuruoka, Yamagata, Japan., Suzuki Y; Department of Earth and Planetary Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Jazyk:	angličtina
Zdroj:	The ISME journal [ISME J] 2024 Jan 08; Vol. 18 (1).
DOI:	10.1093/ismejo/wrae207
Abstrakt:	Recent successes in the cultivation of DPANN archaea with their hosts have demonstrated an episymbiotic lifestyle, whereas the lifestyle of DPANN archaea in natural habitats is largely unknown. A free-living lifestyle is speculated in oxygen-deprived fluids circulated through rock media, where apparent hosts of DPANN archaea are lacking. Alternatively, DPANN archaea may be detached from their hosts and/or rock surfaces. To understand the ecology of rock-hosted DPANN archaea, rocks rather than fluids should be directly characterized. Here, we investigated a deep-sea hydrothermal vent chimney without fluid venting where our previous study revealed the high proportion of Pacearchaeota, one of the widespread and enigmatic lineages of DPANN archaea. Using spectroscopic methods with submicron soft X-ray and infrared beams, the microbial habitat was specified to be silica-filled pores in the inner chimney wall comprising chalcopyrite. Metagenomic analysis of the inner wall revealed the lack of biosynthetic genes for nucleotides, amino acids, cofactors, and lipids in the Pacearchaeota genomes. Genome-resolved metaproteomic analysis clarified the co-occurrence of a novel thermophilic lineage actively fixing carbon and nitrogen and thermophilic archaea in the inner chimney wall. We infer that the shift in metabolically active microbial populations from the thermophiles to the mesophilic DPANN archaea occurs after the termination of fluid venting. The infilling of mineral pores by hydrothermal silica deposition might be a preferred environmental factor for the colonization of free-living Pacearchaeota with ultrasmall cells depending on metabolites synthesized by the co-occurring thermophiles during fluid venting. (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
Databáze:	MEDLINE
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