Saccharomyces boulardii enhances anti-inflammatory effectors and AhR activation via metabolic interactions in probiotic communities.

Autor:	Hedin KA; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby 2800, Denmark., Mirhakkak MH; Department of Microbiome Dynamics, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (Leibniz-HKI), Jena 07745, Germany., Vaaben TH; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby 2800, Denmark., Sands C; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby 2800, Denmark., Pedersen M; National Food Institute, Technical University of Denmark, Kgs. Lyngby 2800, Denmark., Baker A; Human Health Biosolutions, Novonesis, Hørsholm 2970, Denmark., Vazquez-Uribe R; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby 2800, Denmark.; Center for Microbiology, VIB-KU Leuven, Leuven 3001, Belgium., Schäuble S; Department of Microbiome Dynamics, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (Leibniz-HKI), Jena 07745, Germany., Panagiotou G; Department of Microbiome Dynamics, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (Leibniz-HKI), Jena 07745, Germany.; Friedrich Schiller University, Institute of Microbiology, Faculty of Biological Sciences, Jena 07743, Germany.; Friedrich Schiller University, Jena University Hospital, Jena 07743, Germany.; Department of Medicine, University of Hong Kong, Hong Kong, SAR, China., Wellejus A; Human Health Biosolutions, Novonesis, Hørsholm 2970, Denmark., Sommer MOA; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby 2800, Denmark.
Jazyk:	angličtina
Zdroj:	The ISME journal [ISME J] 2024 Nov 03. Date of Electronic Publication: 2024 Nov 03.
DOI:	10.1093/ismejo/wrae212
Abstrakt:	Metabolic exchanges between strains in gut microbial communities shape their composition and interactions with the host. This study investigates the metabolic synergy between potential probiotic bacteria and Saccharomyces boulardii, aiming to enhance anti-inflammatory effects within a multi-species probiotic community. By screening a collection of 85 potential probiotic bacterial strains, we identified two strains that demonstrated a synergistic relationship with S. boulardii in pairwise co-cultivation. Furthermore, we computationally predicted cooperative communities with symbiotic relationships between S. boulardii and these bacteria. Experimental validation of 28 communities highlighted the role of S. boulardii as a key player in microbial communities, significantly boosting the community's cell number and production of anti-inflammatory effectors, thereby affirming its essential role in improving symbiotic dynamics. Based on our observation, one defined community significantly activated the aryl hydrocarbon receptor-a key regulator of immune response-280-fold more effectively than the community without S. boulardii. This study underscores the potential of microbial communities for the design of more effective probiotic formulations. (© The Author(s) [2024]. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
Databáze:	MEDLINE
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