Differential susceptibility to hypoxia in hypoxia-inducible factor 1-alpha (HIF-1α)-targeted freshwater water flea Daphnia magna mutants.

Autor: Lee Y; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea., Byeon E; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea., Lee JS; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea., Maszczyk P; Department of Hydrobiology, Faculty of Biology, University of Warsaw, Warsaw, Poland., Kim HS; School of Pharmacy, Sungkyunkwan University, Suwon 16419, South Korea., Sayed AEH; Department of Zoology, Faculty of Science, Assiut University, Assiut 71516, Egypt., Yang Z; Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, Nanjing 210023, China., Lee JS; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address: jslee2@skku.edu., Kim DH; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address: dhkim5@skku.edu.
Jazyk: angličtina
Zdroj: Marine pollution bulletin [Mar Pollut Bull] 2024 Oct 31; Vol. 209 (Pt B), pp. 117138. Date of Electronic Publication: 2024 Oct 31.
DOI: 10.1016/j.marpolbul.2024.117138
Abstrakt: The water flea, Daphnia magna, serves as a key model organism for investigating the response of aquatic organisms to environmental stressors, including hypoxia. Hypoxia-inducible factor 1-alpha (HIF-1α) is a central regulatory protein involved in the cellular response to hypoxic conditions. In this study, we used CRISPR/Cas9 gene editing to create D. magna mutant lines with targeted alterations in the HIF-1α gene. Mutants demonstrated decreased survival and reproductive output and down-regulated genes for the HIF-1α-mediated pathway in low-oxygen conditions. These findings suggest that the HIF-1α pathway is a critical component of resistance to hypoxia in D. magna. This study provides novel insights into the molecular basis of hypoxia tolerance of HIF-1α in D. magna and expands our understanding of how aquatic organisms can adapt to or be challenged by changing oxygen levels in the face of global environmental changes.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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