F-BAR proteins CIP4 and FBP17 function in cortical neuron radial migration and process outgrowth.

Autor:	English LA; Neuroscience Training Program, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705., Taylor RJ; Neuroscience Department, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705., Cameron CJ; Neuroscience Department, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705., Broker EA; Neuroscience Department, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705., Dent EW; Neuroscience Department, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Oct 25. Date of Electronic Publication: 2024 Oct 25.
DOI:	10.1101/2024.10.25.620310
Abstrakt:	Neurite initiation from newly born neurons is a critical step in neuronal differentiation and migration. Neuronal migration in the developing cortex is accompanied by dynamic extension and retraction of neurites as neurons progress through bipolar and multipolar states. However, there is a relative lack of understanding regarding how the dynamic extension and retraction of neurites is regulated during neuronal migration. In recent work we have shown that CIP4, a member of the F-BAR family of membrane bending proteins, inhibits cortical neurite formation in culture, while family member FBP17 induces premature neurite outgrowth. These results beg the question of how CIP4 and FBP17 function in radial neuron migration and differentiation in vivo , including the timing and manner of neurite extension and retraction. Indeed, the regulation of neurite outgrowth is essential for the transitions between bipolar and multipolar states during radial migration. To examine the effects of modulating expression of CIP4 and FBP17 in vivo , we used in utero electroporation, in combination with our published Double UP technique, to compare knockdown or overexpression cells with control cells within the same mouse tissue of either sex. We show that either knockdown or overexpression of CIP4 and FBP17 results in the marked disruption of radial neuron migration by modulating neuronal morphology and neurite outgrowth, consistent with our findings in culture. Our results demonstrate that the F-BAR proteins CIP4 and FBP17 are essential for proper radial migration in the developing cortex and thus play a key role in cortical development. Competing Interests: CONFLICT OF INTEREST STATEMENT The authors declare no competing financial interests.
Databáze:	MEDLINE
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