Polysaccharides from Gaultheria leucocarpa var. yunnanensis (DBZP) alleviates rheumatoid arthritis through ameliorating gut microbiota.

Autor: Dong Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Wang Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Zhang F; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Ma J; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Li M; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Liu W; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Yao J; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Sun M; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Cao Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Liu Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Ying L; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Yang Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., Yang Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China., She G; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: shegaimei@126.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 281 (Pt 2), pp. 136250. Date of Electronic Publication: 2024 Oct 31.
DOI: 10.1016/j.ijbiomac.2024.136250
Abstrakt: Gaultheria leucocarpa var. yunnanensis (Dianbaizhu) is a traditional Chinese herb for rheumatoid arthritis (RA). However, its macromolecular components have always been overlooked. This study aimed to investigate the chemical composition and effect on improving RA of polysaccharides from Dianbaizhu (DBZP). The results showed the yield of DBZP was 4.07 % ± 0.03 %, and it was composed of Mannose (6.63 %), ribose (1.33 %), rhamnose (4.53 %), glucuronic acid (2.95 %), galacturonic acid (32.29 %), glucose (13.78 %), galactose (22.97 %), xylose (3.94 %) and arabinose (11.59 %), with a large molecular weight distribution range. DBZP treatment could reduce the paws thickness and arthritis scores of collagen-induced arthritis (CIA) mice, and improve inflammatory cell infiltration, synovial hyperplasia, bone erosion, and deterioration. The abundance of several specific bacteria, such as Lactobacillus, Bacteroides, Alistipes, Mucispirillum, and Candidatus_Saccharimonas, and some metabolites in feces or urine, such as 11beta-hydroxytestosterone, pregnanediol 3-O-glucuronide, p-cresol sulfate and several amino acids and peptides, was also altered. The process of DBZP alleviating RA through gut microbiota involves affecting the digestion and metabolism of carbohydrates and protein, altering sex hormones levels, and regulating intestinal immune function, such as the differentiation and signaling of Th17 cells. These findings suggest that DBZP possesses a protective effect on CIA in mice via modulating gut microbiota.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: