Local application of melatonin associated or not to xenogeneic material, in critical defects of rat calvaria.

Autor:	Dalla Costa KL; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil., Lacerda Dias J; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil., Crunivel Araújo IM; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil., Braga Monteiro A; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil., Coelho Junior JA; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil., Scipioni Junior R; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil., Sperandio M; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil., Joly JC; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil., Peruzzo DC; Faculdade e Centro de Pesquisas Odontológicas São Leopoldo Mandic, Campinas, São Paulo, Brasil.
Jazyk:	angličtina
Zdroj:	Acta odontologica latinoamericana : AOL [Acta Odontol Latinoam] 2024 Sep 30; Vol. 37 (2), pp. 123-133.
DOI:	10.54589/aol.37/2/123
Abstrakt:	Melatonin (MLT) is a hormone that can stimulate bone formation and inhibit bone resorption, among other functions. Aim: To evaluate the effect on new bone formation of MLT applied locally to critical defects created in the calvaria of rats, compared to the effect of Bio-Oss® xenogeneic bone substitute (BO), by analyzing histomorphometry, microtomography and gene expression. Materials and Method: Two critical defects (5.0 mm in diameter) were created in the calvaria of 36 adults male Wistar rats. The rats were divided randomly into two groups: a test group, in which one of the defects was filled with MLT, and the other with MLT with Bio-Oss® (MLTBO), and a control group, in which one of the defects was filled only with the clot (C), and the other with BO. The rats were euthanized 30 days after surgery. Samples of the calvaria containing the critical defects were collected for analysis by histomorphometry, microtomography, and the expression of the genes for type I collagen (COL-I), osteopontin (OPN) and bone morphogenetic protein 2 (BMP-2). Results: A qualitative improvement was observed in bone healing when MLT was used, though there was no statistical difference in the quantification of newly formed bone (p>0.05). Micro-CT showed that bone volume was significantly smaller in absence of BO (p=0.006). Bone trabeculae thickness (p=0.590) and number (p=0.150) were not significantly affected by MLT. Regarding the expression of the genes COL-I, OPN and BMP-2, no significant differences were observed between the MLT, BO and MLTBO groups. Conclusion: Topical application of MLT resulted in a qualitative improvement in bone healing, although it did not affect bone formation quantitatively. In the absence of BO, less bone volume and less bone trabecular thickness were observed. Competing Interests: The authors declare no potential conflicts of interest regarding this research or the authorship and/or publication of this article (SAIO.)
Databáze:	MEDLINE
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