The role of neuropeptide prothoracicotropic hormone (PTTH) - Torso in pyriproxyfen-induced larval-pupal abnormal metamorphosis in silkworms.

Autor: He C; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China., Li Y; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China., Zhou Z; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China., Wei Y; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China., Zhu Y; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China., Han Y; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China., Li Y; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China., Yang R; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China., Xu K; College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China; Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi 530004, PR China. Electronic address: kzxu@gxu.edu.cn.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2024 Nov; Vol. 205, pp. 106139. Date of Electronic Publication: 2024 Sep 18.
DOI: 10.1016/j.pestbp.2024.106139
Abstrakt: The neuropeptide prothoracicotropic hormone (PTTH) plays a key role in regulating ecdysone synthesis and promoting insect metamorphosis. Pyriproxyfen is a juvenile hormone analogue. We previously reported that pyriproxyfen disrupts ecdysone secretion and inhibits larval-pupal metamorphosis in silkworms. However, the specific molecular mechanisms by which pyriproxyfen interferes with ecdysone signaling remain to be elucidated. Herein, the RNA-seq analysis on the ecdysone-secretion organ prothoracic gland (PG) was conducted following pyriproxyfen exposure. A total of 3774 differentially expressed genes (DEGs) were identified, with 1667 up-regulated and 2107 down-regulated. KEGG analysis showed that DEGs were enriched in the MAPK signaling pathway, a conserved pathway activated by PTTH binding to Torso, which regulates the ecdysone synthesis. qRT-PCR results indicated a significant up-regulation in PTTH transcription level, while the transcription levels of torso and downstream MAPK pathway genes, Ras2, Raf and ERK, were down-regulated 24 h post-pyriproxyfen treatment. Consistent with these transcriptional changes, PTTH titers in the brain also increased following pyriproxyfen treatment. These results suggest that pyriproxyfen induces abnormal metamorphosis in silkworms by impairing PTTH-Torso signaling. This study enhances our understanding of the molecular mechanisms of pyriproxyfen-induced larval-pupal abnormal metamorphosis in silkworms, and also provides insights for developing detoxification strategies for juvenile hormone analog pesticides to non-target organisms.
Competing Interests: Declaration of competing interest The authors that there are no conflicts of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: