Mutual Interactions of Silymarin and Colon Microbiota in Healthy Young and Healthy Elder Subjects.

Autor: Tomisova K; Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic., Jarosova V; Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic., Marsik P; Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic., Bergo AM; Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic., Cinek O; Department of Pediatrics, Charles University and University Hospital Motol, V Uvalu 84, Prague, 150 06, Czech Republic., Hlinakova L; Department of Pediatrics, Charles University and University Hospital Motol, V Uvalu 84, Prague, 150 06, Czech Republic., Kloucek P; Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic., Janousek V; Biodviser Ltd, 2 Pass St, Oldham, OL9 6HZ, United Kingdom., Valentová K; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, Prague, 142 00, Czech Republic., Havlik J; Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic.
Jazyk: angličtina
Zdroj: Molecular nutrition & food research [Mol Nutr Food Res] 2024 Oct 30, pp. e2400500. Date of Electronic Publication: 2024 Oct 30.
DOI: 10.1002/mnfr.202400500
Abstrakt: Scope: This multi-omic study investigates the bidirectional interactions between gut microbiota and silymarin metabolism, highlighting the differential effects across various age groups. Silymarin, the extract from Silybum marianum (milk thistle), is commonly used for its hepatoprotective effects.
Methods and Results: An in vitro fermentation colon model was used with microbiota from 20 stool samples obtained from healthy donors divided into two age groups. A combination of three analytical advanced techniques, namely proton nuclear magnetic resonance ( 1 H NMR), next-generation sequencing (NGS), and liquid chromatography-mass spectrometry (LC-MS) was used to determine silymarin microbial metabolites over 24 h, overall metabolome, and microbiota composition. Silymarin at a low diet-relevant dose of 50 µg mL -1 significantly altered gut microbiota metabolism, reducing short-chain fatty acid (acetate, butyrate, propionate) production, glucose utilization, and increasing alpha-diversity. Notably, the study reveals age-related differences in silymarin catabolism. Healthy elderly donors (70-80 years) exhibited a significant increase in a specific catabolite associated with Oscillibacter sp., whereas healthy young donors (12-45 years) showed a faster breakdown of silymarin components, particularly isosilybin B, which is associated with higher abundance of Faecalibacterium and Erysipelotrichaceae UCG-003.
Conclusion: This study provides insights into microbiome functionality in metabolizing dietary flavonolignans, highlighting implications for age-specific nutritional strategies, and advancing our understanding of dietary (poly)phenol metabolism.
(© 2024 The Author(s). Molecular Nutrition & Food Research published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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