A multicenter study comparing the bacterial reduction on flexible endoscopes without a working channel between UV-C light disinfection versus standard endoscope Washer Disinfection: a randomized controlled trial.

Autor: Halmans Y; Department of Otorhinolaryngology and Head and Neck Surgery, Radboud University Medical Center Nijmegen, P/O Box 9101, Nijmegen, The Netherlands. yana.halmans@radboudumc.nl.; Radboud University Medical Center Nijmegen, P/O Box 9101, Nijmegen, The Netherlands. yana.halmans@radboudumc.nl., Wellenstein DJ; Department of Otorhinolaryngology and Head and Neck Surgery, Radboud University Medical Center Nijmegen, P/O Box 9101, Nijmegen, The Netherlands., Romijn M; Department of Otorhinolaryngology and Head and Neck Surgery, Radboud University Medical Center Nijmegen, P/O Box 9101, Nijmegen, The Netherlands., van Bemmel AJM; Department of Otorhinolaryngology and Head and Neck Surgery, Medical Spectrum Twente, P/O 50000, Enschede, The Netherlands., van den Berge H; Department of Otorhinolaryngology and Head and Neck Surgery, Medical Center Leeuwarden, P/O 888, Leeuwarden, The Netherlands., Scheeren RA; Department of Otorhinolaryngology, Spaarne Hospital, P/O 417, Haarlem, The Netherlands., Kalpoe JS; Department of Medical Microbiology, Spaarne Hospital, P/O 417, Haarlem, The Netherlands., Klont R; Department of Medical Microbiology, Medical Spectrum Twente, P/O 50000, Enschede, The Netherlands., van Zeijl JH; Department of Medical Microbiology, Medical Center Leeuwarden, P/O 888, Leeuwarden, The Netherlands., Sikkema H; Department of Hopsital Pharmacy, Medical Center Leeuwarden, P/O 888, Leeuwarden, 8901 BR, The Netherlands., Euser SM; Department of Epidemiology and Infection Prevention, Regional Public Health Laboratory Kennemerland, Haarlem, The Netherlands., Hopman J; Department of Medical Microbiology, Radboud University Medical Center Nijmegen, P/O Box 9101, Nijmegen, The Netherlands., Takes RP; Department of Otorhinolaryngology and Head and Neck Surgery, Radboud University Medical Center Nijmegen, P/O Box 9101, Nijmegen, The Netherlands., van den Broek GB; Department of Otorhinolaryngology and Head and Neck Surgery, Radboud University Medical Center Nijmegen, P/O Box 9101, Nijmegen, The Netherlands.
Jazyk: angličtina
Zdroj: Antimicrobial resistance and infection control [Antimicrob Resist Infect Control] 2024 Oct 26; Vol. 13 (1), pp. 128. Date of Electronic Publication: 2024 Oct 26.
DOI: 10.1186/s13756-024-01486-2
Abstrakt: Background: To prevent cross-contamination between patients, adequate reprocessing is necessary when using flexible endoscopes (FEs) without a working channel. The current reprocessing process using an Endoscope Washer Disinfector (EWD) is time-consuming. Ultraviolet light group C (UV-C) exposition is an alternative and fast disinfection method and has previously been shown to adequately reduce Colony Forming Units (CFUs) on FEs without a working channel. The objective of this study was to examine whether UV-C light is as effective in reducing CFUs on contaminated FEs without a working channel compared to the EWD.
Methods: FEs without a working channel were collected in three different Otorhinolaryngology Departments in the Netherlands. After pharyngolaryngoscopy, a manual pre-cleaning with tap water was performed and a culture was collected by rolling the distal 8-10 cm of the FE over an agar plate. Next, the FE was randomly assigned to be disinfected with UV-C light (D60) or the EWD (gold standard). After disinfection, another culture was taken. The primary outcome was microbiological contamination, defined by Colony Forming Units (CFU).
Results: 600 FEs without a working channel were randomized. After clinical use and manual pre-cleaning, 239/300 (79.7%) FEs in the UV-C group and 262/300 (87.3%) FEs in the EWD group were contaminated (i.e., > 0 CFU). FEs without culture confirmed contamination were excluded from further analysis. After UV-C light disinfection, 195/239 (81.6%) FEs showed 0 CFUs, compared to 187/262 (71.4%) FEs disinfected with the EWD (p < 0.01). A multivariate logistics regression analysis showed an increased odds of 0 CFUs when using UV-C light (OR 1.83, 95% CI 1.19-2.79; p < 0.01), conditional on participating hospitals and types of FE.
Conclusions: UV-C light disinfection of FEs without a working channel appears more effective in reducing CFUs compared to the EWD and might be a good alternative disinfection method.
Trial Registration: Not applicable.
(© 2024. The Author(s).)
Databáze: MEDLINE
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