Absolute Quantitative Targeted Monitoring of Potential Plasma Protein Biomarkers: A Pilot Study on Healthy Individuals.

Autor:	Kononikhin AS; Project Center of Advanced Mass Spectrometry Technologies, 121205 Moscow, Russia.; Institute of Biomedical Problems, Russian Federation State Scientific Research Center, Russian Academy of Sciences, 123007 Moscow, Russia., Starodubtseva NL; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, 117997 Moscow, Russia.; Moscow Center for Advanced Studies, 123592 Moscow, Russia., Brzhozovskiy AG; Project Center of Advanced Mass Spectrometry Technologies, 121205 Moscow, Russia.; Institute of Biomedical Problems, Russian Federation State Scientific Research Center, Russian Academy of Sciences, 123007 Moscow, Russia., Tokareva AO; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, 117997 Moscow, Russia., Kashirina DN; Institute of Biomedical Problems, Russian Federation State Scientific Research Center, Russian Academy of Sciences, 123007 Moscow, Russia., Zakharova NV; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia., Bugrova AE; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, 117997 Moscow, Russia.; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia., Indeykina MI; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia., Pastushkova LK; Institute of Biomedical Problems, Russian Federation State Scientific Research Center, Russian Academy of Sciences, 123007 Moscow, Russia., Larina IM; Institute of Biomedical Problems, Russian Federation State Scientific Research Center, Russian Academy of Sciences, 123007 Moscow, Russia., Mitkevich VA; Engelhardt Institute of Molecular Biology, Russian Academy of Science, 119991 Moscow, Russia., Makarov AA; Engelhardt Institute of Molecular Biology, Russian Academy of Science, 119991 Moscow, Russia., Nikolaev EN; Project Center of Advanced Mass Spectrometry Technologies, 121205 Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Biomedicines [Biomedicines] 2024 Oct 21; Vol. 12 (10). Date of Electronic Publication: 2024 Oct 21.
DOI:	10.3390/biomedicines12102403
Abstrakt:	Background/objectives: The development of blood tests for the early detection of individual predisposition to socially significant diseases remains a pressing issue. Methods: In this pilot study, multiple reaction monitoring mass spectrometry (MRM-MS) with a BAK-270 assay was applied for protein concentrations analysis in blood plasma from 21 healthy volunteers of the European cohort. Results: The levels of 138 plasma proteins were reliably and precisely quantified in no less than 50% of samples. The quantified proteins included 66 FDA-approved markers of cardiovascular diseases (CVD), and other potential biomarkers of pathologies such as cancer, diabetes mellitus, and Alzheimer's disease. The analysis of individual variations of the plasma proteins revealed significant differences between the male (11) and female (10) groups. In total, fifteen proteins had a significantly different concentration in plasma; this included four proteins that exhibited changes greater than ±1.5-fold, three proteins (RBP4, APCS, and TTR) with higher levels in males, and one (SHBG) elevated in females. The obtained results demonstrated considerable agreement with the data collected from 20 samples of a North American cohort, which were analyzed with the similar MRM assay. The most significant differences between the cohorts of the two continents were observed in the level of 42 plasma proteins (including 24 FDA markers), of which 17 proteins showed a ≥1.5-fold change, and included proteins increased in North Americans (APOB, CRTAC1, C1QB, C1QC, C9, CRP, HP, IGHG1, IGKV4-1, SERPING1, RBP4, and AZGP1), as well as those elevated in Europeans (APOF, CD5L, HBG2, SELPLG, and TNA). Conclusions: The results suggest a different contribution of specific (patho)physiological pathways (e.g., immune system and blood coagulation) to the development of socially significant diseases in Europeans and North Americans, and they should be taken into account when refining diagnostic panels.
Databáze:	MEDLINE
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