Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos.

Autor: Alkan KK; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye. Electronic address: kubra.alkan@selcuk.edu.tr., Satilmis F; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye., Sonmez G; Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye., Deniz YE; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye., Culha MH; Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye., Ciftci MF; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye., Yesilkaya OF; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye., Alkan H; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2025 Jan 01; Vol. 231, pp. 133-143. Date of Electronic Publication: 2024 Oct 21.
DOI: 10.1016/j.theriogenology.2024.10.017
Abstrakt: The aim of this study was to investigate the effect of putrescine, anti-apoptotic, antioxidant, and a cell proliferation stimulant, on embryo development and quality by supplementing it to in vitro culture medium. In this study, oocytes were obtained from the ovaries of Holstein cattle. Following maturation and fertilization, the presumptive zygotes were randomly assigned to two groups. The first group (Putrescine, n = 435) was supplemented with putrescine at a concentration of 0.5 mM to in vitro culture. The second group (n = 407) was maintained under standard culture conditions without any supplementations to the medium. Following the determination of the developmental stages of the embryos, only those in the blastocyst stage were subjected to differential staining and the cell numbers of the embryos were determined. Moreover, the TUNEL assay was employed to ascertain the extent of cell death and the apoptotic index in the embryos. Additionally, the levels of ROS were determined in the embryos. Furthermore, gene expression analyses were conducted on blastocyst-stage embryos to ascertain the potential of putrescine supplementation in embryo development along specific pathways. Following in vitro culture, the blastocyst formation rate was 44.37 % in the putrescine group and 32.97 % in the control group (P < 0.05). The counts of ICM (60.60 ± 15.79 vs 50.73 ± 16.74), TE (117.70 ± 23.67 vs 94.0 ± 22.46), and TCC (178.30 ± 26.15 vs 144.73 ± 26.86) were found to be statistically higher in blastocysts developing after putrescine supplementation compared to the control group. Furthermore, the number of apoptotic cells (7.69 ± 2.17 vs 9.96 ± 3.99) and the apoptotic index (5.07 % vs 8.01 %) were found to be lower in the putrescine group in comparison to the control group. Nevertheless, it was established that the ROS level in the control group was approximately two-fold higher than in the putrescine group (P < 0.05). The findings also revealed that putrescine up-regulated the gene expression of SOD, GPX4, CAT, BCL2, NANOG and GATA3 while simultaneously down-regulating the BAX expression level. In conclusion, the supplementation of putrescine to the culture medium during in vitro bovine embryo production was found to contribute to the improvement of embryo quality and early embryonic development.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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