Cell Population-resolved Multi-Omics Atlas of the Developing Lung.

Autor:	Ushakumary MG; Pacific Northwest National Laboratory, Biological Sciences Division, Richland, Washington, United States., Feng S; Pacific Northwest National Laboratory, Biological Science Division, Richland, Washington, United States., Bandyopadhyay G; University of Rochester Medical Center, Department of Pediatrics, Rochester, New York, United States., Olson H; Pacific Northwest National Laboratory Biological Sciences Division, Richland, Washington, United States., Weitz KK; Pacific Northwest National Laboratory, 1Biological Sciences Division, Richland, Washington, United States., Huyck HL; University of Rochester Medical Center, Department of Pediatrics, Rochester, New York, United States., Poole C; University of Rochester Medical Center, Department of Pediatrics, Rochester, New York, United States., Purkerson JM; University of Rochester Medical Center, Department of Pediatrics, Rochester, New York, United States., Bhattacharya S; University of Rochester Medical Center, Department of Pediatrics, Rochester, New York, United States., Ljungberg MC; Baylor College of Medicine, Department of Pediatrics, Houston, Texas, United States.; Jan and Dan Duncan Neurological Research Institute, Houston, Texas, United States., Mariani TJ; University of Rochester Medical Center, Department of Pediatrics, Rochester, New York, United States., Deutsch GH; University of Washington, Department of Laboratory Medicine and Pathology, Seattle, Washington, United States., Misra RS; University of Rochester Medical Center, Division of Pediatrics, Rochester, New York, United States., Carson JP; The University of Texas at Austin, Texas Advanced Computing Center, Austin, Texas, United States., Adkins JN; Pacific Northwest National Laboratory, Biological Science Division, Richland, Washington, United States.; Oregon Health & Science University, Department of Biomedical Engineering, Portland, Oregon, United States., Pryhuber GS; University of Rochester Medical Center, Pediatrics, Rochester, New York, United States., Clair G; Pacific Northwest National Laboratory, Biological Science Division, Richland, Washington, United States; geremy.clair@pnnl.gov.
Jazyk:	angličtina
Zdroj:	American journal of respiratory cell and molecular biology [Am J Respir Cell Mol Biol] 2024 Oct 24. Date of Electronic Publication: 2024 Oct 24.
DOI:	10.1165/rcmb.2024-0105OC
Abstrakt:	The lung is a vital organ that undergoes extensive morphological and functional changes during postnatal development. To disambiguate how different cell populations contribute to organ development, we performed proteomic and transcriptomic analyses of four sorted cell populations from the lung of human subjects aged 0 to 8 years-old with a focus on early life. The cell populations analyzed included epithelial, endothelial, mesenchymal, and immune cells. Our results revealed distinct molecular signatures for each of the sorted cell populations that enable the description of molecular shifts occurring in these populations during post-natal development. We confirmed that the proteome of the different cell populations was distinct regardless of age and identified functions specific to each population. We identified a series of cell population protein markers, including those located at the cell surface, that show differential expression and distribution on RNA in situ hybridization and immunofluorescence imaging. We validated the spatial distribution of AT1 and endothelial cell surface markers. Temporal analyses of the proteomes of the four populations revealed processes modulated during postnatal development and clarified the findings obtained from whole tissue proteome studies. Finally, the proteome was compared to a transcriptomics survey performed on the same lung samples to evaluate processes under post-transcriptional control.
Databáze:	MEDLINE
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