Organoid intelligence for developmental neurotoxicity testing.

Autor:	Alam El Din DM; Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States., Shin J; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United States., Lysinger A; Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States., Roos MJ; Research and Exploratory Development Department, Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States., Johnson EC; Research and Exploratory Development Department, Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States., Shafer TJ; Center for Computational Toxicology and Exposure, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC, United States., Hartung T; Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States.; Center for Alternatives to Animal Testing Europe, University of Konstanz, Konstanz, Germany.; Doerenkamp-Zbinden Chair for Evidence-based Toxicology, Baltimore, MD, United States., Smirnova L; Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular neuroscience [Front Cell Neurosci] 2024 Oct 08; Vol. 18, pp. 1480845. Date of Electronic Publication: 2024 Oct 08 (Print Publication: 2024).
DOI:	10.3389/fncel.2024.1480845
Abstrakt:	The increasing prevalence of neurodevelopmental disorders has highlighted the need for improved testing methods to determine developmental neurotoxicity (DNT) hazard for thousands of chemicals. This paper proposes the integration of organoid intelligence (OI); leveraging brain organoids to study neuroplasticity in vitro, into the DNT testing paradigm. OI brings a new approach to measure the impacts of xenobiotics on plasticity mechanisms - a critical biological process that is not adequately covered in current DNT in vitro assays. Finally, the integration of artificial intelligence (AI) techniques will further facilitate the analysis of complex brain organoid data to study these plasticity mechanisms. Competing Interests: Thomas Hartung is named inventor on a patent by Johns Hopkins University on the production of organoids, which is licensed to Axo-Sim, New Orleans, LA, USA. Thomas Hartung and Lena Smirnova are consultants for AxoSim, New Orleans, and Thomas Hartung is also a consultant for AstraZeneca and American Type Culture Collection (ATCC) on advanced cell culture methods. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Alam El Din, Shin, Lysinger, Roos, Johnson, Shafer, Hartung and Smirnova.)
Databáze:	MEDLINE
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