Alginate-modified graphene oxide anchored with lactoperoxidase as a novel bioactive nanocombination for colorectal cancer therapy.

Autor: Nayl AA; Department of Chemistry, College of Science, Jouf University, Sakaka, Aljouf, 72341, Saudi Arabia. aanayel@ju.edu.sa., El-Fakharany EM; Protein Research Department, Genetic Engineering and Biotechnology Research Institute GEBRI, City of Scientific Research and Technological Applications (SRTA city), New Borg El-Arab, Alexandria, 21934, Egypt.; Pharmaceutical and Fermentation Industries Development Centre (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, Alexandria, Egypt.; Pharos University in Alexandria, Canal El Mahmoudia Street, Beside Green Plaza Complex, Alexandria, 21648, Egypt., Abd-Elhamid AI; Composites and Nanostructured Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg Al-Arab, Alexandria, 21934, Egypt., Arafa WAA; Department of Chemistry, College of Science, Jouf University, Sakaka, Aljouf, 72341, Saudi Arabia., Alanazi AH; Department of Chemistry, College of Science, Jouf University, Sakaka, Aljouf, 72341, Saudi Arabia., Ahmed IM; Department of Chemistry, College of Science, Jouf University, Sakaka, Aljouf, 72341, Saudi Arabia., Abdelgawad MA; Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka, 72341, Al Jouf, Saudi Arabia., Aly AA; Chemistry Department, Faculty of Science, Organic Division, Minia University, El-Minia, 61519, Egypt., Bräse S; Institute of Biological and Chemical Systems - Functional Molecular Systems (IBCS-FMS), Kaiserstrasse 12, Karlsruhe, 76131, Germany. stefan.braese@kit.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 22; Vol. 14 (1), pp. 24804. Date of Electronic Publication: 2024 Oct 22.
DOI: 10.1038/s41598-024-74604-0
Abstrakt: It is imperative to explore new biocompatible drugs with low toxicity for use in medicinal fields such as fighting tumors. Bovine lactoperoxidase (BLPO) stems from the most important enzymes in the bovine whey that provide a proper pattern for nano-formulation with nanomaterials. LPO is a suitable protein to be coated or adsorbed to alginate modified graphene oxide (GO-SA), which forms the modified GO-SA-LPO hybrid structure. This novel combination provides LPO stability with strong anticancer effects and boosts immunity response. The characterization results obtained from different techniques confirmed a successful LPO adsorption on the GO-SA composite surface. Moreover, nano-formulation of LPO with GO-SA composite exhibited a reduction in its size and overall charge. In addition, the experimental results showed greater LPO activity stability in the modified GO-SA-LPO nanocombination than free LPO after storage for 10 weeks at 4 °C. The in vitro study, a crucial step in the validation of our approach, demonstrated that the modified GO-SA-LPO nanocombination showed a potent anticancer selectivity toward colon cancer cell lines more than GO-SA composite or free form of LPO, which enhanced in a dose-dependent manner with high safety manner against normal cells. The apoptotic effect of this novel nanocombination was confirmed by the greatest variations in the expression of both well-known apoptosis genes (p53 and Bcl-2), severe changes in the cellular morphology, DNA fragmentation, and nuclear staining with fluorescence yellow and orange of the target cancer cells. Also, this superior efficacy of the modified GO-SA-LPO nanocombination was induced by suppressing some pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin (IL-6), and necrosis factor-kappa B (NF-ĸB). Our observations presented that the modified nanocombination of LPO may offer a novel remedy for treating colon tumors via induced apoptosis pathway, inflammation reduction, and immune response improvement.
(© 2024. The Author(s).)
Databáze: MEDLINE
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