Assay variables and early clinical evaluation of low-angle light scattering for platelet function analysis.

Autor: Svidelskaya GS; National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation.; Faculty of Physics, M. V. Lomonosov Moscow State University, Moscow, Russian Federation.; Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Moscow, Russian Federation., Sorkina VP; National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation., Ignatova AA; National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation., Ponomarenko EA; National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation., Poletaev AV; National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation., Seregina EA; National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation., Manuvera VA; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russian Federation.; Moscow Institute of Physics and Technology, Dolgoprudny, Russian Federation., Zharkov PA; National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation., Mindukshev IV; Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russian Federation., Gambaryan S; Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russian Federation., Panteleev MA; National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation. mapanteleev@yandex.ru.; Faculty of Physics, M. V. Lomonosov Moscow State University, Moscow, Russian Federation. mapanteleev@yandex.ru.; Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Moscow, Russian Federation. mapanteleev@yandex.ru.
Jazyk: angličtina
Zdroj: International journal of hematology [Int J Hematol] 2024 Dec; Vol. 120 (6), pp. 717-724. Date of Electronic Publication: 2024 Oct 22.
DOI: 10.1007/s12185-024-03859-0
Abstrakt: Introduction: The recently developed platelet aggregation technique based on low-angle light scattering (LaSca) in diluted platelet-rich plasma (PRP) requires only a small sample volume and provides information about platelet aggregation and shape change. This study aimed to investigate the influence of preanalytical and analytical variables and to validate the method in a real-life pediatric hematology hospital setting.
Methods: Platelet aggregation was induced by ADP in diluted PRP in the presence of 2 mM calcium at 23 °C. The study included healthy adults (n = 30), healthy children (n = 20), and pediatric patients with suspected or diagnosed platelet function abnormalities (n = 25).
Results: The assay parameters were stable for at least 3 h after isolation of PRP and were sensitive to plasma dilution in the range of 2-8%. The initial aggregation velocity was significantly reduced in pediatric patients compared with healthy children (p < 0.05). ADP-induced light transmission amplitude was moderately correlated with LaSca amplitude of aggregation in healthy children (p = 0.52, p < 0.05) but not in pediatric patients.
Conclusions: We standardized the protocol for platelet aggregation assessment by LaSca and characterized the influence of preanalytical and analytical variables on it.
Competing Interests: Declarations. Conflict of interest: The authors declare that there is no conflict of interest. Institutional review board statement: The study was conducted in accordance with the Declaration of Helsinki, and approved by the Ethics Committee of the Center for Theoretical Problems of Physicochemical Pharmacology (protocol № 2/1–20 from 03.08.2020). Informed consent: Informed consent was obtained from all subjects involved in the study.
(© 2024. Japanese Society of Hematology.)
Databáze: MEDLINE
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