Novel compound, pleuropyronine, and other polyketides isolated from the edible mushroom Pleurotus ostreatus suppress bacterial biofilm formation.

Autor:	De Padua JC; The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan., Kikuchi T; Rigaku Corporation, Akishima-shi, Tokyo, Japan.; Novel Compounds Exploration Research Organization, Tsukuba, Japan., Sakakibara F; Novel Compounds Exploration Research Organization, Tsukuba, Japan., De Leon AM; Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines., Bungihan ME; Department of Chemistry, College of Science, University of Santo Tomas, España Blvd., Manila, Philippines.; Research Center for the Natural and Applied Sciences, University of Santo Tomas, España Blvd., Manila, Philippines., Ueno K; Department of Agricultural, Life, Environmental Sciences, Faculty of Agriculture, Tottori University, Tottori, Japan., Dela Cruz TEE; Department of Biological Sciences, College of Science, University of Santo Tomas, España Blvd., Manila, Philippines., Ishihara A; Novel Compounds Exploration Research Organization, Tsukuba, Japan.; Department of Agricultural, Life, Environmental Sciences, Faculty of Agriculture, Tottori University, Tottori, Japan.
Jazyk:	angličtina
Zdroj:	Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2024 Dec 23; Vol. 89 (1), pp. 11-21.
DOI:	10.1093/bbb/zbae148
Abstrakt:	An increase in the number of drug-resistant microbes is a major threat to human health. Bacterial drug resistance is mostly mediated by biofilm formation. In this study, the culture filtrate from the edible mushroom, Pleurotus ostreatus, was fractionated to isolate compounds that inhibit the biofilm formation of six pathogenic bacteria. Notably, we isolated compounds 1-6 using bioassay-guided chromatographic separations. Spectroscopic and X-ray diffraction analyses identified 1 as a novel fused bicyclic pyrone-furan, named pleuropyronine, whereas 2-6 were known polyketides. Pleuropyronine inhibited biofilm formation in four Gram-negative bacteria, with IC50 values ranging from 5.4 to 8.7 µg/mL, whereas 2-6 exhibited IC50 values between 1.0 and 5.3 µg/mL against five bacteria. Additionally, pleuropyronine bioactivity was confirmed by the inhibition of exopolysaccharide and biofilm formation induced by C6-homoserine lactone. Thus, this may serve as a pioneering study on the pharmacological potential of isolated compounds, offering valuable insights for future research. (The Author(s) 2024. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
Databáze:	MEDLINE
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