Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.
| Autor: | Olie SE; Department of Neurology, Amsterdam UMC, University of Amsterdam, Amsterdam Neuroscience, Amsterdam, the Netherlands., Andersen CØ; Statens Serum Institute, Diagnostic Infectious Disease Preparedness, Copenhagen, Denmark., van de Beek D; Department of Neurology, Amsterdam UMC, University of Amsterdam, Amsterdam Neuroscience, Amsterdam, the Netherlands., Brouwer MC; Department of Neurology, Amsterdam UMC, University of Amsterdam, Amsterdam Neuroscience, Amsterdam, the Netherlands. |
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| Jazyk: | angličtina |
| Zdroj: | Clinical microbiology reviews [Clin Microbiol Rev] 2024 Dec 10; Vol. 37 (4), pp. e0002124. Date of Electronic Publication: 2024 Oct 15. |
| DOI: | 10.1128/cmr.00021-24 |
| Abstrakt: | SUMMARYCentral nervous system (CNS) infections can be caused by various pathogens, including bacteria, viruses, fungi, and parasites. Molecular diagnostic methods are pivotal for identifying the different causative pathogens of these infections in clinical settings. The efficacy and specificity of these methods can vary per pathogen involved, and in a substantial part of patients, no pathogen is identified in the cerebrospinal fluid (CSF). Over recent decades, various molecular methodologies have been developed and applied to patients with CNS infections. This review provides an overview of the accuracy of nucleic acid amplification methods in CSF for a diverse range of pathogens, examines the potential value of multiplex PCR panels, and explores the broad-range bacterial and fungal PCR/sequencing panels. In addition, it evaluates innovative molecular approaches to enhance the diagnosis of CNS infections. Competing Interests: The authors declare no conflict of interest. |
| Databáze: | MEDLINE |
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