Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.

Autor: Roeterink RMA; Department of Biomedical Engineering, The University of Melbourne, VIC, Parkville, Victoria, 3010, Australia.; Department of Biosystems - MeBioS, KU Leuven, Willem de Croylaan 42, Leuven, 3001, Belgium.; The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC, 3052, Australia., Casadevall I Solvas X; Department of Biosystems - MeBioS, KU Leuven, Willem de Croylaan 42, Leuven, 3001, Belgium., Collins DJ; Department of Biomedical Engineering, The University of Melbourne, VIC, Parkville, Victoria, 3010, Australia.; The Graeme Clark Institute, The University of Melbourne, Parkville, VIC, 3010, Australia., Scott DJ; The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC, 3052, Australia.; Department of Biochemistry and Pharmacology, The University of Melbourne, Parkville, VIC, 3010, Australia.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Oct 14, pp. e2402167. Date of Electronic Publication: 2024 Oct 14.
DOI: 10.1002/adhm.202402167
Abstrakt: Mechanotransduction is the process whereby cells convert mechanical signals into electrochemical responses, where mechanosensitive proteins mediate this interaction. To characterize these critical proteins, numerous techniques have been developed that apply forces and measure the subsequent cellular responses. While these approaches have given insight into specific aspects of many such proteins, subsequent validation and cross-comparison between techniques remain difficult given significant variations in reported activation thresholds and responses for the same protein across different studies. Accurately determining mechanosensitivity responses for various proteins, however, is essential for understanding mechanotransduction and potential physiological implications, including therapeutics. This critical review provides an assessment of current and emerging approaches used for mechanosensitive ion channel and G-Coupled Receptors (GPCRs) stimulation and measurement, with a specific focus on the ability to quantitatively measure mechanosensitive responses.
(© 2024 The Author(s). Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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