| Autor: |
Schottdorf M; Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA., Rich PD; Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA., Diamanti EM; Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA., Lin A; Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA.; Center for the Physics of Biological Function, Princeton University, Princeton, NJ, USA., Tafazoli S; Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA., Nieh EH; Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA.; School of Medicine, University of Virginia, Charlottesville, VA, USA., Thiberge SY; Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA.; Bezos Center for Neural Circuit Dynamics, Princeton University, Princeton, NJ, USA. |
| Abstrakt: |
Many laboratories use two-photon microscopy through commercial suppliers, or homemade designs of considerable complexity. The integrated nature of these systems complicates customization, troubleshooting as well as grasping the principles of two-photon microscopy. Here, we present "Twinkle": a microscope for Two-photon Imaging in Neuroscience, and Kit for Learning and Education. It is a fully open, high-performance and cost-effective research and teaching microscope without any custom parts beyond what can be fabricated in a university machine shop. The instrument features a large field of view, using a modern objective with a long working distance and large back aperture to maximize the fluorescence signal. We document our experiences using this system as a teaching tool in several two week long workshops, exemplify scientific use cases, and conclude with a broader note on the place of our work in the growing space of open-source scientific instrumentation. |
