The hemostatic activity and Mechanistic roles of glucosyloxybenzyl 2-isobutylmalate extract (BSCE) from Bletilla striata (Thunb.) Rchb.f. in Inhibiting pulmonary hemorrhage.

Autor:	Liu G; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Mu KL; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Ran F; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Liu JM; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Zhou LL; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Peng LQ; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Feng G; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Liu YC; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Wei FD; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Zhu LL; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Zhang XY; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Zhang YP; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China., Sun QW; Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, China.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Sep 20; Vol. 10 (18), pp. e38203. Date of Electronic Publication: 2024 Sep 20 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e38203
Abstrakt:	Background: Hemorrhagic events cause numerous deaths annually worldwide, highlighting the urgent need for effective hemostatic drugs. The glucosyloxybenzyl 2-isobutylmalates Control Extract (BSCE) from the orchid plant Bletilla striata (Thunb.) Rchb.f. has demonstrated significant hemostatic activity in both in vitro and in vivo studies. However, the effect and mechanism of BSCE on non-traumatic bleeding remain unclear. Methods: Pulmonary hemorrhage was induced in 40 Sprague-Dawley rats by administering Zingiber officinale Roscoe. for 14 days. These rats were then randomly divided into five groups: model (Mod), positive control (YNBY), and BSCE low, medium, and high-dose groups. An additional 8 rats served as the control group (Con). The BSCE groups received different doses of BSCE for 10 days, while the YNBY group received Yunnan Baiyao suspension. The effects on body weight, food and water intake, red blood cell count (RBC), hemoglobin concentration (HGB), lung tissue pathology, platelet count, coagulation parameters, and fibrinolytic system markers were evaluated. Network pharmacology and molecular docking analyses were also conducted to identify potential targets and pathways involved in BSCE's effects. Results: BSCE treatment significantly improved body weight, food intake, and water consumption in rats with pulmonary hemorrhage. RBC and HGB levels increased significantly in the BSCE medium and high-dose groups compared to the Mod group (P < 0.05). Pathological examination revealed that BSCE reduced lung tissue hemorrhage and inflammation, with improvements in alveolar structure. BSCE also positively affected platelet count, thrombin time (TT), activated partial thromboplastin time (APTT), fibrinogen (FIB) levels, and fibrinolytic markers (D-dimer, PAI-1, and t-PA). Network pharmacology and molecular docking identified key targets such as MMPs, CASPs, and pathways including IL-17 and TNF signaling, suggesting BSCE's involvement in hemostasis and anti-inflammatory processes. Conclusions: BSCE exhibits significant hemostatic and protective effects on Z . officinale -induced pulmonary hemorrhage in rats by improving hematological parameters, reducing lung tissue damage, and modulating the coagulation and fibrinolytic systems. The study provides evidence supporting the potential of BSCE as a therapeutic agent for hemorrhagic diseases, with its efficacy linked to multi-target and multi-pathway interactions. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Author(s).)
Databáze:	MEDLINE
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