The Role of Bovine Amniotic Membrane and Hydroxyapatite for the Ridge Preservation.

Autor: Octarina O; Department of Dental Material, Faculty of Dentistry, Universitas Trisakti, Jakarta 11440, Indonesia.; Doctoral Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia., Munadziroh E; Department of Dental Material, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia., Razak FA; Department of Oral and Craniofacial Sciences, Faculty of Dentistry, Universiti Malaya, Kuala Lumpur 50603, Malaysia., Handharyani E; Division of Pathology, School of Veterinary Medicine and Biomedical, Institute Pertanian Bogor University, Bogor 16680, Indonesia., Surboyo MDC; Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia.
Jazyk: angličtina
Zdroj: International journal of biomaterials [Int J Biomater] 2024 Sep 14; Vol. 2024, pp. 4053527. Date of Electronic Publication: 2024 Sep 14 (Print Publication: 2024).
DOI: 10.1155/2024/4053527
Abstrakt: Ridge preservation is an important technique for maintaining the dimensions of the alveolar bone following tooth extraction, which is crucial for successful tooth rehabilitation. The combination of bovine amniotic membrane and hydroxyapatite has shown promise as a scaffold material containing growth factors that can stimulate osteogenic-related factors such as bone morphogenetic protein 2 (BMP2), Runt-related transcription factor 2 (RUNX2), and osteocalcin. This stimulation leads to collagen production and osteoblast proliferation, resulting in new bone formation. In this study, bovine amniotic membrane-hydroxyapatite (BAM-HA) composites were prepared using three different ratios of bovine amniotic membrane and hydroxyapatite (2 : 3, 3 : 7, 7 : 13). Thirty Sprague-Dawley rats had their first incisors extracted, and different types of BAM-HA were applied for ridge preservation. The control group received no treatment, while the positive control group was given xenograft. After 14 and 28 days, the animals were sacrificed, and immunohistochemical analysis was performed to evaluate the expression of BMP2, RUNX2, and osteocalcin. Additionally, a histological examination was conducted to analyse collagen thickness and osteoblast cell proliferation. The results demonstrated that the application of BAM-HA significantly increased collagen density, osteoblast cell proliferation, and the expression of BMP2, RUNX2, and osteoclacin compared to the control group ( p < 0.05) on both days 14 and 28. Furthermore, increasing the hydroxyapatite content in the composite was found to enhance collagen thickness, osteoblast cell proliferation, and the expression of osteogenic-related factors. These preliminary findings suggest that the combination of BAM-HA can be used for ridge preservation to prevent further bone resorption following tooth extraction.
Competing Interests: The authors declare that they no conflicts of interest.
(Copyright © 2024 Octarina Octarina et al.)
Databáze: MEDLINE
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