Comparative transcriptional analysis between susceptible and resistant populations of Aedes (Stegomyia) aegypti (Linnaeus, 1762) after malathion exposure.

Autor: de Souza RF; Laboratório de Bioinformática, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil., Amaro TR; Laboratório de Citogenética e Entomologia Molecular, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid/Pr 445 Km 380, Londrina, Paraná, CEP: 86057-970, Brazil., Palacio-Cortés AM; Laboratório de Morfologia e Fisiologia de Culicidae E Chironomidae, Departamento de Zoologia, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Brazil., da Silva MAN; Laboratório de Morfologia e Fisiologia de Culicidae E Chironomidae, Departamento de Zoologia, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Brazil., Dionisio JF; Laboratório de Citogenética e Entomologia Molecular, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid/Pr 445 Km 380, Londrina, Paraná, CEP: 86057-970, Brazil., Pezenti LF; Laboratório de Citogenética e Entomologia Molecular, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid/Pr 445 Km 380, Londrina, Paraná, CEP: 86057-970, Brazil., Lopes TBF; Laboratório de Citogenética e Entomologia Molecular, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid/Pr 445 Km 380, Londrina, Paraná, CEP: 86057-970, Brazil., Mantovani MS; Laboratório de Genética Toxicológica, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil., Zequi JAC; Laboratório de Entomologia Médica, Departamento de Biologia Animal e Vegetal, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil., da Rosa R; Laboratório de Citogenética e Entomologia Molecular, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid/Pr 445 Km 380, Londrina, Paraná, CEP: 86057-970, Brazil. renata-darosa@uel.br.
Jazyk: angličtina
Zdroj: Molecular genetics and genomics : MGG [Mol Genet Genomics] 2024 Oct 05; Vol. 299 (1), pp. 92. Date of Electronic Publication: 2024 Oct 05.
DOI: 10.1007/s00438-024-02185-8
Abstrakt: Aedes aegypti is an important vector of arboviruses, including dengue, chikungunya and Zika. The application of synthetic insecticides is a frequently used strategy to control this insect. Malathion is an organophosphate insecticide that was widely used in Brazil in the 1980s and 1990s to control the adult form of A. aegypti. In situations where resistance to currently used insecticides is detected, the use of malathion may be resumed as a control measure. Many studies have confirmed resistance to malathion, however, comparative studies of differential gene expression of the entire transcriptome of resistant and susceptible insects are scarce. Therefore, understanding the molecular basis of resistance to this insecticide in this species is extremely important. In this paper, we present the first transcriptomic description of susceptible and resistant strains of A. aegypti challenged with malathion. Guided transcriptome assembly resulted in 39,904 transcripts, where 2133 differentially expressed transcripts were detected, and three were validated by RT-qPCR. Enrichment analysis for these identified transcripts resulted in 13 significant pathways (padj < 0.05), 8 associated with down-regulated and 5 with up-regulated transcripts in treated resistant insects. It was possible to divide the transcripts according to the mechanism of action into three main groups: (i) genes involved in detoxification metabolic pathways; (ii) genes of proteins located in the membrane/extracellular region; and (iii) genes related to DNA integration/function. These results are important in advancing knowledge of genes related to resistance mechanisms in this insect, enabling the development of effective technologies and strategies for managing insecticide resistance.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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