LAPONITE® nano-silicates potentiate the angiogenic effects of FG-4592 and osteogenic effects of BMP-2.

Autor:	Gaihre B; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA. Lu.Lichun@mayo.edu.; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, 55905, USA., Camilleri E; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA. Lu.Lichun@mayo.edu.; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, 55905, USA., Tilton M; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA. Lu.Lichun@mayo.edu.; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, 55905, USA., Astudillo Potes MD; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA. Lu.Lichun@mayo.edu.; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, 55905, USA., Liu X; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA. Lu.Lichun@mayo.edu.; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, 55905, USA., Lucien F; Department of Urology, Mayo Clinic, Rochester, MN, 55905, USA.; Department of Immunology, Mayo Clinic, Rochester, MN, 55905, USA., Lu L; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA. Lu.Lichun@mayo.edu.; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, 55905, USA.
Jazyk:	angličtina
Zdroj:	Biomaterials science [Biomater Sci] 2024 Oct 22; Vol. 12 (21), pp. 5610-5619. Date of Electronic Publication: 2024 Oct 22.
DOI:	10.1039/d4bm00636d
Abstrakt:	LAPONITE®-based drug delivery systems offer many advantages due to the unique ionic and physical properties of LAPONITE®. The high ionicity and large surface area of LAPONITE® nanoparticles enable the intercalation and dissolution of biomolecules. In this study, we explored the potential of LAPONITE® as a carrier for FG-4592 to support angiogenesis and as a carrier for bone morphogenic protein-2 (BMP-2) to support osteogenesis. Interestingly, we found that LAPONITE® promoted the FG-4592 induced upregulation of vascular endothelial growth factor (VEGF) gene expression of human umbilical cord endothelial cells (HUVECs). Additionally, we observed that LAPONITE® could provide a sustained release of BMP-2 and significantly potentiate the osteogenic effects of BMP-2 on adipose derived mesenchymal stem cells (AMSCs). Overall, current findings on the LAPONITE®-drug/protein model system provide a unique way to potentiate the angiogenic activities of FG-4592 on HUVECs and osteogenic effects of BMP-2 on AMSCs for tissue engineering application. Future studies will be directed towards gaining a deeper understanding of these effects on a co-culture system of HUVECs and AMSCs.
Databáze:	MEDLINE
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