Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor.

Autor: Lokareddy RK; Department of Biochemistry and Molecular Genetics, University of Alabama at. Birmingham (UAB), 1825 University Blvd, Birmingham, AL, USA., Hou CD; Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ, USA., Forti F; Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy., Iglesias SM; Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA., Li F; Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA., Pavlenok M; Department of Microbiology, University of Alabama at Birmingham, 845 19th Street South, Birmingham, AL, USA., Horner DS; Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy., Niederweis M; Department of Microbiology, University of Alabama at Birmingham, 845 19th Street South, Birmingham, AL, USA., Briani F; Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy. federica.briani@unimi.it., Cingolani G; Department of Biochemistry and Molecular Genetics, University of Alabama at. Birmingham (UAB), 1825 University Blvd, Birmingham, AL, USA. gcingola@uab.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Oct 01; Vol. 15 (1), pp. 8482. Date of Electronic Publication: 2024 Oct 01.
DOI: 10.1038/s41467-024-52752-1
Abstrakt: DEV is an obligatory lytic Pseudomonas phage of the N4-like genus, recently reclassified as Schitoviridae. The DEV genome encodes 91 ORFs, including a 3398 amino acid virion-associated RNA polymerase (vRNAP). Here, we describe the complete architecture of DEV, determined using a combination of cryo-electron microscopy localized reconstruction, biochemical methods, and genetic knockouts. We built de novo structures of all capsid factors and tail components involved in host attachment. We demonstrate that DEV long tail fibers are essential for infection of Pseudomonas aeruginosa but dispensable for infecting mutants with a truncated lipopolysaccharide devoid of the O-antigen. We determine that DEV vRNAP is part of a three-gene operon conserved in 191 Schitoviridae genomes. We propose these three proteins are ejected into the host to form a genome ejection motor spanning the cell envelope. We posit that the design principles of the DEV ejection apparatus are conserved in all Schitoviridae.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: