Sublingual immune cell clusters and dendritic cell distribution in the oral cavity.

Autor:	Kusumoto Y; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Ueda M; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Hashimoto M; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Takeuchi H; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Okada N; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Yamamoto J; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Nishii A; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Fujino A; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Kurahashi A; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Satoh M; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Iwasa Y; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Okamura K; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Obazaki K; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Kumagai R; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Sakamoto N; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Tanaka Y; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Kamiya Y; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Hoshida T; Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, Saitama, Japan., Kaisho T; Department of Immunology, Institute of Advanced Medicine, Wakayama Medical University, Wakayama, Japan., Hemmi H; Department of Immunology, Institute of Advanced Medicine, Wakayama Medical University, Wakayama, Japan., Katakai T; Department of Immunology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan., Honda T; Department of Dermatology, Kyoto University, Graduate School of Medicine, Kyoto, Japan., Kikuta J; Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Japan., Kataoka K; Department of Oral Health Science and Social Welfare, Graduate School of Oral Sciences, Tokushima University, Tokushima, Japan., Ikebuchi R; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Moriya T; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan., Adachi T; Department of Precision Health, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan., Watanabe T; Laboratory of Immunology, Institute for Life and Medical Sciences, Kyoto Universtity, Kyoto, Japan., Ishii M; Laboratory of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Japan., Miyawaki A; Laboratory for Cell Function Dynamics, RIKEN Brain Science Instituite, Saitama, Japan., Kabashima K; Department of Dermatology, Kyoto University, Graduate School of Medicine, Kyoto, Japan., Chtanova T; Immunology Theme, Garvan Institute of Medical Research, Darlinghurst, Australia., Tomura M; Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan.
Jazyk:	angličtina
Zdroj:	JCI insight [JCI Insight] 2024 Oct 01. Date of Electronic Publication: 2024 Oct 01.
DOI:	10.1172/jci.insight.167373
Abstrakt:	The oral mucosa is the first line of defense against pathogenic bacteria and plays a vital role in maintaining tolerance to food antigens and commensal bacteria. We used CD11c reporter mice to visualize dendritic cells (DCs), a key immune cell population, in the oral cavity. We identified differences in DC density in each oral tissue region. Sublingual immune cell clusters (SLICs) extended from the lamina propria to the epithelium, where DCs and T cells resided in close contact with each other and innate lymphoid cells (ILCs). Targeted in situ photolabeling revealed that the SLICs comprised mostly of CD11c+CD11b+ DCs and were enriched for cDC1s and Langerhans cells. Although the frequency of T cell subsets was similar within and outside the SLICs, tissue resident memory T cells were significantly enriched within the clusters and cluster size increased in response to inflammation. Collectively, we found that SLICs form a unique microenvironment that facilitates T cell-DC interactions in the steady state and during inflammation. Since the oral mucosa is an important target for needle-free vaccination and sublingual immunotherapy to induce tolerogenic responses, the novel insight into the localized immunoregulation provided in this study may accelerate the development of these approaches.
Databáze:	MEDLINE
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