Transcriptome meta-analysis of Kawasaki disease in humans and mice.

Autor:	Gu W; Department of Medicine, University of California, San Diego, CA, United States., Mirsaidi-Madjdabadi S; Department of Medicine, University of California, San Diego, CA, United States., Ramirez F Jr; Department of Medicine, University of California, San Diego, CA, United States.; Center for Inflammation Science and Systems Medicine, The Herbert Wertheim University of Florida Scripps Institute for Biomedical Innovation & Technology, Jupiter, FL, United States., Simonson TS; Department of Medicine, University of California, San Diego, CA, United States., Makino A; Department of Medicine, University of California, San Diego, CA, United States.; Center for Inflammation Science and Systems Medicine, The Herbert Wertheim University of Florida Scripps Institute for Biomedical Innovation & Technology, Jupiter, FL, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in pediatrics [Front Pediatr] 2024 Sep 16; Vol. 12, pp. 1423958. Date of Electronic Publication: 2024 Sep 16 (Print Publication: 2024).
DOI:	10.3389/fped.2024.1423958
Abstrakt:	Kawasaki Disease (KD) affects young children less than five years old with severe blood vessel inflammation. Despite being treatable, the causes and mechanisms remain elusive. This study conducted a meta-analysis of RNA sequencing (RNA-seq) data from human and animal models to explore KD's transcriptomic profile and evaluate animal models. We retrieved bulk and single-cell RNA-seq data from Gene Expression Omnibus, with blood and coronary artery samples from KD patients, aorta samples from KD mouse models ( Lactobacillus casei cell wall extract-injected mice), and their controls. Upon consistent quality control, we applied Fisher's exact test to assess differential gene expression, followed by an enrichment analysis of overlapping genes. These studies identified 400 differentially expressed genes in blood samples of KD patients compared to controls and 413 genes in coronary artery samples. The data from KD blood and KD coronary artery samples shared only 16 differentially expressed genes. Eighty-one genes overlapped between KD human coronary arteries and KD mouse aortas, and 67 of these 81 genes were regulated in parallel in both humans and mice: 30 genes were up-regulated, and 37 were down-regulated. These included previously identified KD-upregulated genes: CD74 , S FRP4 , ITGA4 , and IKZF1 . Gene enrichment analysis revealed significant alterations in the cardiomyopathy pathway. Single-cell RNAseq showed a few significant markers, with known KD markers like S100A9 , S100A8 , CD74 , CD14 , IFITM2 , and IFITM3 , being overexpressed in KD cohorts. Gene profiles obtained from KD human coronary artery are more compatible with data from aorta samples of KD mice than blood samples of KD humans, validating KD animal models for identifying therapeutic targets. Although blood samples can be utilized to discover novel biomarkers, more comprehensive single-cell sequencing is required to detail gene expression in different blood cell populations. This study identifies critical genes from human and mouse tissues to help develop new treatment strategies for KD. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2024 Gu, Mirsaidi-Madjdabadi, Ramirez, Simonson and Makino.)
Databáze:	MEDLINE
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