Alpha B-crystallin Ameliorates Imbalance of Redox Homeostasis, Inflammation and Apoptosis in an Acute Lung Injury Model with Rats.

Autor:	Kocak S; Kirsehir Ahi Evran University Faculty of Medicine, Department of Physiology, Kirsehir, Türkiye., Guner I; Tekirdag Namik Kemal University Faculty of Medicine, Department of Physiology, Tekirdag, Türkiye., Yaman MO; Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Physiology, Istanbul, Türkiye., Ekiz Yilmaz T; Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Histology and Embriology, Istanbul, Türkiye., Guzel Meydanli EE; Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Histology and Embriology, Istanbul, Türkiye., Yelmen N; Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Physiology, Istanbul, Türkiye., Sahin G; Istanbul Aydin University Faculty of Medicine, Department of Physiology, Istanbul, Türkiye.
Jazyk:	angličtina
Zdroj:	Medeniyet medical journal [Medeni Med J] 2024 Sep 30; Vol. 39 (3), pp. 211-220.
DOI:	10.4274/MMJ.galenos.2024.82274
Abstrakt:	Objective: Ischemia-reperfusion (IR) of the aorta is a significant contributor to the development of postoperative acute lung damage after abdominal aortic surgery. The aim of the present study was to examine the effect of alpha B-crystallin, a small heat shock protein (known as HspB5), on lung injury induced by abdominal aortic IR in rats. Methods: Male Sprague-Dawley rats were divided into three groups: control, ischemia-reperfusion (IR, 90 min ischemia and 180 min reperfusion), and alpha B-crystallin +IR. Alpha B-crystallin (50 μg/100 g) was intraperitoneally administered 1 h before IR. Lung tissue samples were obtained for histological and biochemical analyses of oxidative stress and cytokine and apoptosis parameters in plasma, lung tissues, and bronchoalveolar lavage (BAL) fluid. Results: The levels of malondialdehyde, reactive oxygen species, total oxidant status, tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), nuclear factor kappa B (NFKβ), caspase-9 (CASP-9), 8-hydroxy-2'-deoxyguanosine, total antioxidant status, superoxide dismutase, and interleukin-10 levels in lung tissues, plasma, and BAL fluid (p<0.05 versus control) increased in Aortic IR. However, alpha B-crystallin significantly reduced the lung tissue levels of oxidative, inflamatuvar, and apoptotic parameters in the plasma, lung tissues, and BAL fluid (p<0.05 versus aortic IR). Histopathological results showed that alpha B-crystallin ameliorated the morphological changes related to lung injury (p<0.001). Conclusion: Alpha B-crystallin substantially restored disrupted the redox balance, inflammation, and apoptotic parameters in rats exposed to IR. The cytoprotective effect of alpha B-crystallin on redox balance might be attributed to improved lung injury. Competing Interests: Conflict of Interest: The authors have no conflict of interest to declare. (Copyright© 2024 The Author. Published by Galenos Publishing House on behalf of Istanbul Medeniyet University Faculty of Medicine.)
Databáze:	MEDLINE
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