Novel multiphoton intravital imaging enables real-time study of Helicobacter pylori interaction with neutrophils and macrophages in the mouse stomach.

Autor: Ishikawa-Ankerhold H; Department of Internal Medicine I, LMU University Hospital, Munich, Germany.; Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, LMU University Hospital, Munich, Germany.; German Centre for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany., Busch B; Chair of Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich, Germany., Bader A; Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, LMU University Hospital, Munich, Germany.; Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-Universität München, Munich, Germany., Maier-Begandt D; Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, LMU University Hospital, Munich, Germany.; Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-Universität München, Munich, Germany., Dionisio F; Department of Internal Medicine I, LMU University Hospital, Munich, Germany., Namineni S; Chair of Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich, Germany., Vladymyrov M; Data Science Lab, Mathematical Institute, University of Bern, Bern, Switzerland., Harrison U; Chair of Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich, Germany., van den Heuvel D; Department of Internal Medicine I, LMU University Hospital, Munich, Germany.; Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, LMU University Hospital, Munich, Germany., Tomas L; Department of Internal Medicine I, LMU University Hospital, Munich, Germany.; German Centre for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany., Walzog B; Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, LMU University Hospital, Munich, Germany.; Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-Universität München, Munich, Germany., Massberg S; Department of Internal Medicine I, LMU University Hospital, Munich, Germany.; German Centre for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany., Schulz C; Department of Internal Medicine I, LMU University Hospital, Munich, Germany.; German Centre for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany.; Department of Immunopharmacology, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany., Haas R; German Centre for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, Munich, Germany.; German Center for Infection Research (DZIF), LMU Munich, Germany.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2024 Sep 30; Vol. 20 (9), pp. e1012580. Date of Electronic Publication: 2024 Sep 30 (Print Publication: 2024).
DOI: 10.1371/journal.ppat.1012580
Abstrakt: Helicobacter pylori (H. pylori) is a bacterial pathogen that exclusively colonizes the human gastric mucosa and can cause persistent infection. In this process, H. pylori employs various strategies to avoid recognition by the human immune system. These range from passive defense strategies (e.g., altered LPS or flagellin structures) that prevent recognition by pattern recognition receptors (PRRs) to more active approaches, such as inhibition of IL-2 secretion and proliferation of T cells via VacA. Despite the growing evidence that H. pylori actively manipulates the human immune system for its own benefit, the direct interaction of H. pylori with immune cells in situ is poorly studied. Here, we present a novel intravital imaging model of the murine stomach gastric mucosa and show for the first time the in situ recruitment of neutrophils during infection and a direct H. pylori-macrophage interaction. For this purpose, we applied multiphoton intravital microscopy adapted with live drift correction software (VivoFollow) on LysM-eGFP and CX3CR1-eGFP reporter mice strains in which specific subsets of leukocytes are fluorescently labeled. Multiphoton microscopy is proving to be an excellent tool for characterizing interactions between immune cells and pathogens in vivo.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Ishikawa-Ankerhold et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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