The transcriptomic landscape of spinal V1 interneurons reveals a role for En1 in specific elements of motor output.

Autor:	Trevisan AJ; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA., Han K; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA., Chapman P; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA., Kulkarni AS; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA., Hinton JM; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA., Ramirez C; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA., Klein I; Department of Neurology, University Hospital of Cologne, Cologne, 50937, Germany., Gatto G; Department of Neurology, University Hospital of Cologne, Cologne, 50937, Germany., Gabitto MI; Allen Institute for Brain Science, Seattle, WA, 98109, USA.; Department of Statistics, University of Washington, Seattle, WA, 98109, USA., Menon V; Department of Neurology, Center for Translational and Computational Neuroimmunology, Columbia University, New York, NY, 10033, USA., Bikoff JB; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.; Lead Contact.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Oct 26. Date of Electronic Publication: 2024 Oct 26.
DOI:	10.1101/2024.09.18.613279
Abstrakt:	Neural circuits in the spinal cord are composed of diverse sets of interneurons that play crucial roles in shaping motor output. Despite progress in revealing the cellular architecture of the spinal cord, the extent of cell type heterogeneity within interneuron populations remains unclear. Here, we present a single-nucleus transcriptomic atlas of spinal V1 interneurons across postnatal development. We find that the core molecular taxonomy distinguishing neonatal V1 interneurons perdures into adulthood, suggesting conservation of function across development. Moreover, we identify a key role for En1, a transcription factor that marks the V1 population, in specifying one unique subset of V1 Pou6f2 interneurons. Loss of En1 selectively disrupts the frequency of rhythmic locomotor output but does not disrupt flexion/extension limb movement. Beyond serving as a molecular resource for this neuronal population, our study highlights how deep neuronal profiling provides an entry point for functional studies of specialized cell types in motor output. Competing Interests: DECLARATION OF INTERESTS The authors declare no competing interests.
Databáze:	MEDLINE
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