Rubisco packaging and stoichiometric composition of a native β-carboxysome.

Autor: Sun Y; Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom., Sheng Y; Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom., Ni T; Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, United Kingdom., Ge X; Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom., Sarsby J; Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Crown Street, L69 7ZB, Liverpool, United Kingdom., Brownridge PJ; Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Crown Street, L69 7ZB, Liverpool, United Kingdom., Li K; College of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, China., Hardenbrook N; Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom., Dykes GF; Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom., Rockliffe N; GeneMill, University of Liverpool, Faculty of Health & Life Sciences, University of Liverpool, Crown Street, L69 7ZB, Liverpool, United Kingdom., Eyers CE; Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Crown Street, L69 7ZB, Liverpool, United Kingdom., Zhang P; Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.; Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, United Kingdom.; Chinese Academy of Medical Sciences Oxford Institute, University of Oxford, Oxford, OX3 7BN, United Kingdom., Liu LN; Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, United Kingdom.; College of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, China.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2024 Sep 21. Date of Electronic Publication: 2024 Sep 21.
DOI: 10.1101/2024.09.20.614183
Abstrakt: Carboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation in cyanobacteria. This self-assembling proteinaceous organelle encapsulates the key CO 2 -fixing enzymes, Rubisco and carbonic anhydrase, using a polyhedral shell constructed by hundreds of shell protein paralogs. Deciphering the precise arrangement and structural organization of Rubisco enzymes within carboxysomes is crucial for understanding the formation process and overall functionality of carboxysomes. Here, we employed cryo-electron tomography and subtomogram averaging to delineate the three-dimensional packaging of Rubiscos within β-carboxysomes in the freshwater cyanobacterium Synechococcus elongatus PCC7942 that were grown under low light. Our results revealed that Rubiscos are arranged in multiple concentric layers parallel to the shell within the β-carboxysome lumen. We also identified the binding of Rubisco with the scaffolding protein CcmM in β-carboxysomes, which is instrumental for Rubisco encapsulation and β-carboxysome assembly. Using QconCAT-based quantitative mass spectrometry, we further determined the absolute stoichiometric composition of the entire β-carboxysome. This study and recent findings on the β-carboxysome structure provide insights into the assembly principles and structural variation of β-carboxysomes, which will aid in the rational design and repurposing of carboxysome nanostructures for diverse bioengineering applications.
Competing Interests: Competing Interests The authors declare no conflict of interest.
Databáze: MEDLINE
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