Evaluation of a New In Chemico Skin Corrosion Test.

Autor: Lebrun S; Lebrun Labs LLC, 3301 E. Miraloma Ave., Ste. 194, Anaheim, CA 92806, USA. Electronic address: SJ@lebrunlabs.com., Nguyen L; Lebrun Labs LLC, 3301 E. Miraloma Ave., Ste. 194, Anaheim, CA 92806, USA., Ho KV; Lebrun Labs LLC, 3301 E. Miraloma Ave., Ste. 194, Anaheim, CA 92806, USA.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2024 Dec; Vol. 101, pp. 105944. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1016/j.tiv.2024.105944
Abstrakt: The purpose of this study is to evaluate a novel macromolecular test method for the identification of dermal corrosives. The simple in chemico test procedure involves allowing the material to be tested to interact with a skin biomarker for corrosivity and then adding a detection reagent. The corrosivity of the test substance is predicted based on the measured macromolecular damage, which results in reduced optical density of the detection reagent as compared with controls. This study aims to determine if such an extremely simple, cell-free test method can accurately identify dermal corrosives. To determine predictivity and repeatability, we tested 60 chemicals (30 in vivo dermal corrosives and 30 in vivo dermal noncorrosives; all tested in triplicate) representative of a broad range of chemical classes, functional groups, mixtures, and levels of toxicity. Validation results indicate the GHS multicategory and packing group assignment accuracy is on par with that of the Reconstructed Human Epidermis test method and the Membrane Barrier test method and for the global identification of corrosives, the method has a considerably higher accuracy (98 % vs. ∼80 %).
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Supported by a NIEHS Small Business Innovative Research Grant. Research reported in this publication was supported by the National Institute of Environmental Health Sciences of the National Institutes of Health under Award Number R44ES036065. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Stewart Lebrun reports a relationship with Lebrun Labs that includes: board membership, equity or stocks, and funding grants. Stewart Lebrun and Linda Nguyen are inventors for patent #PCT/US24/15781 IN CHEMICO TEST FOR TOXICITY; pending and assigned to Lebrun Labs LLC. The DermaSafe Skin Corrosion test is sold by InChemico LLC, a wholly owned subsidiary of Lebrun Labs LLC. Stewart Lebrun owns Lebrun Labs LLC. Linda Nguyen and Kelly Ve Ho are Lebrun Labs LLC employees.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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