Non-Ionic Fluorosurfactants for Droplet-Based in vivo Applications.

Autor: van de Wouw HL; Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.; Present address: School of Chemistry and Biochemistry, Georgia Institute of Technology, 770 State Street N.W., Atlanta, GA, 30332, USA., Yen ST; Cluster of Excellence Physics of Life, TU Dresden, Arnoldstrasse 18, 01307, Dresden, Germany.; Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA., Valet M; Cluster of Excellence Physics of Life, TU Dresden, Arnoldstrasse 18, 01307, Dresden, Germany., Garcia JA; Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA., Gomez CO; Cluster of Excellence Physics of Life, TU Dresden, Arnoldstrasse 18, 01307, Dresden, Germany.; Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA., Vian A; Cluster of Excellence Physics of Life, TU Dresden, Arnoldstrasse 18, 01307, Dresden, Germany.; Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA., Liu Y; Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA., Pollock J; Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA., Pospíšil P; Cluster of Excellence Physics of Life, TU Dresden, Arnoldstrasse 18, 01307, Dresden, Germany., Campàs O; Cluster of Excellence Physics of Life, TU Dresden, Arnoldstrasse 18, 01307, Dresden, Germany.; Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.; Max Planck Institute of Molecular Cell Biology and Genetics, Center for Systems Biology Dresden, Pfotenhauerstrasse 108, 01307, Dresden, Germany., Sletten EM; Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2024 Sep 27, pp. e202404956. Date of Electronic Publication: 2024 Sep 27.
DOI: 10.1002/anie.202404956
Abstrakt: Fluorocarbon oils are uniquely suited for many biomedical applications due to their inert, bioorthogonal properties. In order to interface fluorocarbon oils with biological systems, non-ionic fluorosurfactants are necessary. However, there is a paucity of non-ionic fluorosurfactants with low interfacial tension (IFT) to stabilize fluorocarbon phases in aqueous environments (such as oil-in-water emulsions). We developed non-ionic fluorosurfactants composed of a polyethylene glycol (PEG) segment covalently bonded to a flexible perfluoropolyether (PFPE) segment that confer low IFTs between a fluorocarbon oil (HFE-7700) and water. The synthesis of a panel of surfactants spanning a molecular weight range of 0.64-66 kDa with various hydrophilic-lipophilic balances allowed for identification of minimal IFTs, ranging from 1.4 to 17.8 mN m -1 . The majority of these custom fluorosurfactants display poor solubility in water, allowing their co-introduction with fluorocarbon oils and minimal leaching. We applied the PEG 5 PFPE 1 surfactant for mechanical force measurements in zebrafish, enabling exceptional sensitivity.
(© 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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