| Autor: |
Peterle VM; Department of Veterinary Medicine, University of Vila Velha (UVV), Vila Velha 29102-920, ES, Brazil., Cardoso JAB; Department of Veterinary Medicine, University of Vila Velha (UVV), Vila Velha 29102-920, ES, Brazil., Ferraz CM; Department of Veterinary Medicine, University of Vila Velha (UVV), Vila Velha 29102-920, ES, Brazil., Sousa DF; Department of Veterinary Medicine, University of Vila Velha (UVV), Vila Velha 29102-920, ES, Brazil., Pereira N; Department of Pathology, Reproduction and One Health, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil., Nassar AFC; Instituto Biológico (IB) de São Paulo, Rua Conselheiro Rodrigues Alves, 1252, São Paulo 04014-002, SP, Brazil., Castro V; Instituto Biológico (IB) de São Paulo, Rua Conselheiro Rodrigues Alves, 1252, São Paulo 04014-002, SP, Brazil., Mathias LA; Department of Pathology, Reproduction and One Health, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil., Cardozo MV; Department of Pathology, Reproduction and One Health, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil., Rossi GAM; Department of Veterinary Medicine, University of Vila Velha (UVV), Vila Velha 29102-920, ES, Brazil. |
| Abstrakt: |
This study aimed to evaluate the microbiological quality of coconut water sold from street carts equipped with cooling coils or refrigerated at bakeries in the Grande Vitória Region, Brazil. Additionally, it assessed the phenotypic and genotypic antimicrobial resistance profiles of isolated enterobacteria. The results indicated that coconut water sold at street carts had lower microbiological quality compared to refrigerated samples, as evidenced by significantly higher counts of mesophilic microorganisms. Using MALDI-TOF, the following opportunistic pathogens were identified: Citrobacter freundii , Enterobacter bugandensis , E. kobei , E. roggenkampii , Klebsiella pneumoniae , and Kluyvera ascorbata . Three isolates- E. bugandensis , K. pneumoniae , and K. ascorbata -were classified as multidrug-resistant (MDR). Widespread resistance to β-lactams and cephalosporins was detected, and some isolates were resistant to quinolones, nitrofurans, and phosphonic acids. The gene bla CTX-M-2 was detected in C. freundii , E. bugandensis , E. kobei , and K. ascorbata . However, genes bla NDM , bla KPC , bla CMY-1 , and bla CMY-2 were not detected in any isolate. The findings underscore the need to enhance good manufacturing practices in this sector to control the spread of antimicrobial resistance (AMR). To our knowledge, this is the first study documenting the presence of potentially pathogenic enterobacteria in coconut water samples and their associated phenotypic and genotypic AMR profiles. |
