Towards geospatially-resolved public-health surveillance via wastewater sequencing.

Autor:	Tierney BT; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA. btt4001@med.cornell.edu.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA. btt4001@med.cornell.edu., Foox J; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Ryon KA; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Butler D; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Damle N; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Young BG; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Mozsary C; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Babler KM; Department of Human Genetics, University of Utah, Salt Lake City, UT, USA.; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL, USA., Yin X; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL, USA., Carattini Y; Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL, USA., Andrews D; Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL, USA., Lucaci AG; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA., Solle NS; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA., Kumar N; Department of Public Health Sciences, University of Miami Miller School of Medicine, Miami, FL, USA., Shukla B; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA., Vidović D; Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, USA.; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA., Currall B; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA., Williams SL; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA., Schürer SC; Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, USA.; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA.; Institute for Data Science & Computing, University of Miami, Coral Gables, FL, USA., Stevenson M; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA., Amirali A; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL, USA., Beaver CC; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA.; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA., Kobetz E; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA.; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA., Boone MM; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA., Reding B; Environmental Health and Safety, University of Miami, Miami, FL, USA., Laine J; Environmental Health and Safety, University of Miami, Miami, FL, USA., Comerford S; Environmental Health and Safety, University of Miami, Miami, FL, USA., Lamar WE; Division of Occupational Health, Safety & Compliance, University of Miami Health System, Miami, FL, USA., Tallon JJ Jr; Facilities and Operations, University of Miami, Coral Gables, FL, USA., Wain Hirschberg J; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Proszynski J; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Al Ghalith G; Seed Health, Venice, CA, USA., Can Kurt K; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Sharkey ME; Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL, USA., Church GM; Harvard Medical School and the Wyss Institute, Boston, MA, USA., Grills GS; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA., Solo-Gabriele HM; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL, USA. hmsolo@miami.edu., Mason CE; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA. chm2042@med.cornell.edu.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA. chm2042@med.cornell.edu.; The WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY, USA. chm2042@med.cornell.edu.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2024 Sep 27; Vol. 15 (1), pp. 8386. Date of Electronic Publication: 2024 Sep 27.
DOI:	10.1038/s41467-024-52427-x
Abstrakt:	Wastewater is a geospatially- and temporally-linked microbial fingerprint of a given population, making it a potentially valuable tool for tracking public health across locales and time. Here, we integrate targeted and bulk RNA sequencing (N = 2238 samples) to track the viral, bacterial, and functional content over geospatially distinct areas within Miami Dade County, USA, from 2020-2022. We used targeted amplicon sequencing to track diverse SARS-CoV-2 variants across space and time, and we found a tight correspondence with positive PCR tests from University students and Miami-Dade hospital patients. Additionally, in bulk metatranscriptomic data, we demonstrate that the bacterial content of different wastewater sampling locations serving small population sizes can be used to detect putative, host-derived microorganisms that themselves have known associations with human health and diet. We also detect multiple enteric pathogens (e.g., Norovirus) and characterize viral diversity across sites. Moreover, we observed an enrichment of antimicrobial resistance genes (ARGs) in hospital wastewater; antibiotic-specific ARGs correlated to total prescriptions of those same antibiotics (e.g Ampicillin, Gentamicin). Overall, this effort lays the groundwork for systematic characterization of wastewater that can potentially influence public health decision-making. (© 2024. The Author(s).)
Databáze:	MEDLINE
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