Dietary "Beigeing" Fat Contains More Phosphatidylserine and Enhances Mitochondrial Function while Counteracting Obesity.

Autor:	Zhou Y; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou, Zhejiang 310058, China., Ling D; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou, Zhejiang 310058, China., Wang L; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou, Zhejiang 310058, China., Xu Z; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou, Zhejiang 310058, China., You W; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou, Zhejiang 310058, China., Chen W; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou, Zhejiang 310058, China., Nong Q; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou, Zhejiang 310058, China., Valencak TG; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China., Shan T; College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.; Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou, Zhejiang 310058, China.
Jazyk:	angličtina
Zdroj:	Research (Washington, D.C.) [Research (Wash D C)] 2024 Sep 26; Vol. 7, pp. 0492. Date of Electronic Publication: 2024 Sep 26 (Print Publication: 2024).
DOI:	10.34133/research.0492
Abstrakt:	Activation of mitochondrial function and heat production in adipose tissue by the modification of dietary fat is a promising strategy against obesity. However, as an important source of lipids for ketogenic and daily diets, the function of fats extracted from different adipose tissue sites was largely unknown. In this study, we illustrated the function of fats extracted from adipose tissues with different "beigeing" properties in the ketogenic diet and identified lipid profiles of fats that facilitate energy expenditure. We found that the anti-obesity effect of ketogenic diets was potentiated by using "beigeing" fat [porcine subcutaneous adipose tissue (SAT)] as a major energy-providing ingredient. Through lipidomic analyses, phosphatidylserine (PS) was identified as a functional lipid activating thermogenesis in adipose tissue. Moreover, in vivo studies showed that PS induces adipose tissue thermogenesis and alleviates diet-induced obesity in mice. In vitro studies showed that PS promotes UCP1 expression and lipolysis of adipocytes. Mechanistically, PS promoted mitochondrial function in adipocytes via the ADCY3-cAMP-PKA-PGC1α pathway. In addition, PS-PGC1a binding may affect the stability of the PGC1α protein, which further augments PS-induced thermogenesis. These results demonstrated the efficacy of dietary SAT fats in diminishing lipid accumulation and the underlying molecular mechanism of PS in enhancing UCP1 expression and mitochondrial function. Thus, our findings suggest that as dietary fat, "beigeing" fat provides more beneficial lipids that contribute to the improvement of mitochondrial function, including PS, which may become a novel, nonpharmacological therapy to increase energy expenditure and counteract obesity and its related diseases. Competing Interests: Competing interests: The authors declare that they have no competing interests. (Copyright © 2024 Yanbing Zhou et al.)
Databáze:	MEDLINE
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