Reactive oxygen species-inducing itraconazole and its anti-biofilm activity against resistant Candida parapsilosis sensu lato biofilm cells isolated from patients with recalcitrant onychomycosis.

Autor: Kamali M; Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Rafsanjani Hwy, No.1115119969, +982123921, Tehran, Iran., Ghaderi A; School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Tamimi P; School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Firooz A; Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Taleqani Ave. No.415, Tehran, 1416613675, Iran., Nasiri Kashani M; Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Taleqani Ave. No.415, Tehran, 1416613675, Iran., Ayatollahi A; Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Taleqani Ave. No.415, Tehran, 1416613675, Iran., Valizadeh F; Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Keshavarz Blvd No.15, Tehran, +982188962128, Iran., Fattahi M; Mohebe Kowsar Hospital, Tehran, Iran., Fattahi M; Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. dr.mahsafattahi@gmail.com.; Pediatric Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. dr.mahsafattahi@gmail.com.
Jazyk: angličtina
Zdroj: Archives of dermatological research [Arch Dermatol Res] 2024 Sep 26; Vol. 316 (9), pp. 642. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1007/s00403-024-03382-6
Abstrakt: Candida parapsilosis was introduced as the second most responsible for nail involvement. The colonization of biotic and abiotic surfaces by Candida spp. can result in the formation of biofilms, which possess a high level of resistance to typical antifungal agents. Since Candida spp. can produce biofilm mass on the surface of the nails, dermatologists should consider appropriate antifungals to eliminate both the planktonic and biofilm cells. The aim of this research was to determine the antifungal efficacy of itraconazole against C. parapsilosis sensu lato biofilm formations, in addition to its static effects. Ten C. parapsilosis sensu lato isolates were enrolled in this study. The use of itraconazole results in the accumulation of reactive oxygen species (ROS) during treatment. In order to verify the correlation between ROS and itraconazole-induced cell death, the viability of cells was analyzed by administering the ROS scavenger Ascorbic acid. The apoptotic features of itraconazole were analyzed using the Annexin V-FITC method. Based on current data, it was found that the generation of intracellular stresses by itraconazole is not observed in cells upon ROS inhibition, emphasizing the importance of intracellular ROS in the apoptotic mechanism of itraconazole. Targeting the oxidative defense system is a powerful point to use ROS-inducing antifungals as a superior choice for more effective therapies in case of recalcitrant onychomycosis.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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