Sensitive and reliable lab-on-paper biosensor for label-free detection of exosomes by electrochemical impedance spectroscopy.

Autor: Sazaklioglu SA; Faculty of Pharmacy, Department of Analytical Chemistry, Ankara Medipol University, 06050, Ankara, Turkey.; Graduate School of Natural and Applied Science, Gazi University, 06560, Ankara, Turkey., Torul H; Faculty of Pharmacy, Department of Analytical Chemistry, Gazi University, 06330, Ankara, Turkey., Tamer U; Faculty of Pharmacy, Department of Analytical Chemistry, Gazi University, 06330, Ankara, Turkey.; METU MEMS Center, Ankara, Turkey., Ensarioglu HK; Faculty of Medicine, Department of Histology and Embryology, Manisa Celal Bayar University, 45200, Manisa, Turkey., Vatansever HS; Faculty of Medicine, Department of Histology and Embryology, Manisa Celal Bayar University, 45200, Manisa, Turkey.; DESAM Institute, Near East University, Mersin 10, Turkey., Gumus BH; Faculty of Medicine, Department of Urology, Manisa Celal Bayar University, 45200, Manisa, Turkey., Çelikkan H; Faculty of Science, Department of Chemistry, Gazi University, 06560, Ankara, Turkey. celikkan@gazi.edu.tr.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Sep 24; Vol. 191 (10), pp. 617. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1007/s00604-024-06644-2
Abstrakt: A new, sensitive, and cost-effective lab-on-paper-based immunosensor was designed based on electrochemical impedance spectroscopy (EIS) for the detection of exosomes. EIS was selected as the determination method since there was a surface blockage in electron transfer by binding the exosomes to the transducer. Briefly, the carbon working electrode (WE) on the paper electrode (PE) was modified with gold particles (AuPs@PE) and then conjugated with anti-CD9 (Anti-CD9/AuPs@PE) for the detection of exosomes. Variables involved in the biosensor design were optimized with the univariate mode. The developed method presents the limit of detection of  8.7 × 10 2 exosomes mL -1 , which is lower than that of many other available methods under the best conditions. The biosensor was also tested with urine samples from cancer patients with high recoveries. Due to this  a unique, low-cost, biodegradable technology is presented that can directly measure exosomes without labeling them for early cancer or metastasis detection.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: