| Autor: |
Neuhoff MJ; Department of Physics, The Ohio State University, Columbus, Ohio 43210, United States., Wang Y; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, United States., Vantangoli NJ; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, United States., Poirier MG; Department of Physics, The Ohio State University, Columbus, Ohio 43210, United States.; Biophysics Graduate Program, The Ohio State University, Columbus, Ohio 43210, United States.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, United States., Castro CE; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, United States.; Biophysics Graduate Program, The Ohio State University, Columbus, Ohio 43210, United States., Pfeifer WG; Department of Physics, The Ohio State University, Columbus, Ohio 43210, United States.; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, United States. |
| Abstrakt: |
DNA origami nanotechnology has great potential in multiple fields including biomedical, biophysical, and nanofabrication applications. However, current production pipelines lead to single-use devices incorporating a small fraction of initial reactants, resulting in a wasteful manufacturing process. Here, we introduce two complementary approaches to overcome these limitations by recycling the strand components of DNA origami nanostructures (DONs). We demonstrate reprogramming entire DONs into new devices, reusing scaffold strands. We validate this approach by reprogramming DONs with complex geometries into each other, using their distinct geometries to verify successful scaffold recycling. We reprogram one DON into a dynamic structure and show both pristine and recycled structures display similar properties. Second, we demonstrate the recovery of excess staple strands postassembly and fold DONs with these recycled strands, showing these structures exhibit the expected geometry and dynamic properties. Finally, we demonstrate the combination of both approaches, successfully fabricating DONs solely from recycled DNA components. |
