The markers of the predictive DNA test for canine hip dysplasia may have a stronger relationship with elbow dysplasia.

Autor:	Ardicli S; Bursa Uludag University, Faculty of Veterinary Medicine, Department of Genetics, Bursa, Turkey.; Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland., Yigitgor P; Bursa Uludag University, Faculty of Veterinary Medicine, Department of Surgery, Bursa, Turkey., Babayev H; Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland., Ozen D; Ankara University, Faculty of Veterinary Medicine, Department of Biostatistics, Ankara, Turkey., Bozkurt B; Department of Biotechnology and Bioengineering, Graduate School of Science and Engineering, Izmir Institute of Technology, Izmir, Turkey., Senturk N; Bursa Uludag University, Faculty of Veterinary Medicine, Department of Genetics, Bursa, Turkey., Pilli M; Near East University, Faculty of Veterinary Medicine, Department of Surgery, Nicosia, Cyprus., Salci H; Bursa Uludag University, Faculty of Veterinary Medicine, Department of Surgery, Bursa, Turkey., Seyrek Intas D; Near East University, Faculty of Veterinary Medicine, Department of Surgery, Nicosia, Cyprus.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Sep 10; Vol. 10 (18), pp. e37716. Date of Electronic Publication: 2024 Sep 10 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e37716
Abstrakt:	Canine hip and elbow dysplasias, which are prevalent orthopedic conditions rooted in developmental and hereditary factors are yet to be comprehensively assessed. This study aimed to address this gap by exploring the prognostic significance of five markers linked to canine hip dysplasia using available genome-wide association studies (GWAS) data. The influence of these markers on both hip and elbow dysplasia was examined in dogs exposed to standardized environmental conditions. We made a groundbreaking discovery using custom primers, qPCR assays, and evaluation of fluorescent resonance energy transfer (FRET) probes. Three specific SNPs previously associated with the risk of canine hip dysplasia demonstrated a potentially stronger correlation with elbow dysplasia. Notably, the SNP at nucleotide position 22691322, located near the canine CHST3 gene, displayed significance as a marker in multivariable logistic regression analysis. Surprisingly, none of the initially targeted SNPs showed a direct association with hip dysplasia. The genomic positions of these SNPs reside within a region conserved across mammals. In silico analyses suggested that the relevant variant might be positioned in a region linked to bone and muscle structures. Our findings revealed a remarkable relationship between SNP2 genotypes and methylation patterns, shedding light on the underlying mechanism that partially explains the genotype-phenotype correlation in canine CHST3 . These groundbreaking findings offer essential insights for future, more extensive investigations into canine orthopedic health. This research significantly contributes to our understanding of the molecular foundations of hip and elbow dysplasia in dogs by charting a course for advancements in veterinary medicine and the overall well-being of canine companions. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu