The Arabidopsis U1 snRNP regulates mRNA 3'-end processing.

Autor: Mangilet AF; Institute of Biology and Environmental Sciences, University of Oldenburg, Oldenburg, Germany.; Max Planck Institute for Plant Breeding Research (MPIPZ), Cologne, Germany., Weber J; Institute of Biology and Environmental Sciences, University of Oldenburg, Oldenburg, Germany.; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Schüler S; Institute of Biology and Environmental Sciences, University of Oldenburg, Oldenburg, Germany.; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Adler M; Institute of Biology and Environmental Sciences, University of Oldenburg, Oldenburg, Germany.; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Mjema EY; Institute of Biology and Environmental Sciences, University of Oldenburg, Oldenburg, Germany.; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Heilmann P; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Herold A; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Renneberg M; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Nagel L; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Droste-Borel I; Proteome Center, University of Tuebingen, Tuebingen, Germany., Streicher S; Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Schmutzer T; Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Rot G; Institute of Molecular Life Sciences of the University of Zurich and Swiss Institute of Bioinformatics, Zurich, Switzerland., Macek B; Proteome Center, University of Tuebingen, Tuebingen, Germany., Schmidtke C; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Laubinger S; Institute of Biology and Environmental Sciences, University of Oldenburg, Oldenburg, Germany. sascha.laubinger@genetik.uni-halle.de.; Institute of Biology, Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany. sascha.laubinger@genetik.uni-halle.de.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2024 Oct; Vol. 10 (10), pp. 1514-1531. Date of Electronic Publication: 2024 Sep 23.
DOI: 10.1038/s41477-024-01796-8
Abstrakt: The removal of introns by the spliceosome is a key gene regulatory mechanism in eukaryotes, with the U1 snRNP subunit playing a crucial role in the early stages of splicing. Studies in metazoans show that the U1 snRNP also conducts splicing-independent functions, but the lack of genetic tools and knowledge about U1 snRNP-associated proteins have limited the study of such splicing-independent functions in plants. Here we describe an RNA-centric approach that identified more than 200 proteins associated with the Arabidopsis U1 snRNP and revealed a tight link to mRNA cleavage and polyadenylation factors. Interestingly, we found that the U1 snRNP protects mRNAs against premature cleavage and polyadenylation within introns-a mechanism known as telescripting in metazoans-while also influencing alternative polyadenylation site selection in 3'-UTRs. Overall, our work provides a comprehensive view of U1 snRNP interactors and reveals novel functions in regulating mRNA 3'-end processing in Arabidopsis, laying the groundwork for understanding non-canonical functions of plant U1 snRNPs.
(© 2024. The Author(s).)
Databáze: MEDLINE
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