Investigation of synergistic interaction of sinensetin, eupatorin, and 3'-hydroxy-5,6,7,4'-tetramethoxyflavone in vasodilation efficacy.

Autor: Yam MF; College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China. yammunfei@yahoo.com.; Department of Chinese Medicine, M Kandiah Faculty of Medicine & Health Sciences, Universiti Tunku Abdul Rahman, Kajang, Selangor, Malaysia. yammunfei@yahoo.com.; Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang, Malaysia. yammunfei@yahoo.com., Tew WY; Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang, Malaysia., Tan CS; Material Characterization and Inorganic Spectroscopy, Perkin Elmer Sdn Bhd, Wisma Academy, Petaling Jaya, Selangor, Malaysia., Qiu Q; Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang, Malaysia., Zhou R; Department of Acupuncture and Moxibustion, Affiliated Hospital of Guizhou Medical University, Guiyang, China., Wang X; Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang, Malaysia., Yap YP; Department of Chinese Medicine, M Kandiah Faculty of Medicine & Health Sciences, Universiti Tunku Abdul Rahman, Kajang, Selangor, Malaysia., Xu W; College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China., Xu W; College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China. 2012029@fjtcm.edu.cn., Teh LK; Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia. tehlk@utar.edu.my.
Jazyk: angličtina
Zdroj: Hypertension research : official journal of the Japanese Society of Hypertension [Hypertens Res] 2024 Nov; Vol. 47 (11), pp. 3193-3199. Date of Electronic Publication: 2024 Sep 19.
DOI: 10.1038/s41440-024-01907-0
Abstrakt: Modern medicines often follow a "single-compound, single-target" paradigm, which may not be effective against complex diseases with multifactorial causes. Medicinal plants, such as Orthosiphon stamineus-widely used in Southeast Asia for its significant vasodilatory and antihypertensive properties-offer an alternative. These effects are largely attributed to the synergistic actions of sinensetin, eupatorin, and 3'-hydroxy-5,6,7,4'- tetramethoxyflavone (TMF). The present study was designed to explore the interactions among these compounds and their collective impact on vasodilation. The current investigation utilized in vitro aortic ring assays and an orthogonal stimulus-response compatibility approach to unveil the synergistic interactions of sinensetin, eupatorin, and TMF in specific combination ratios within compatibility groups. The current results showed that G2, G7, G27, and G28 achieved vasodilatory efficacies exceeding 100%, with recorded efficacies of 190%, 148%, 117.6%, and 116.25%, respectively. Conversely, formulation F1 exhibited only additive effects with an efficacy of 88.02%. The dose-response study revealed G28 exhibited the strongest concentration-dependent vasodilatory responses, with a maximum response (R MAX ) of 119.05 ± 3.29% and an EC 50 of 6.78 ± 0.70 µg/mL. Conversely, G2, despite showing the highest efficacy in the orthogonal stimulus-response compatibility study, demonstrated a lower vasodilatory effect, with R MAX R and EC 50 recorded at 85.78 ± 12.67% and 15.32 ± 3.07 µg/mL, respectively. These findings highlight the complexities of compound interactions in plants and underscore the potential of botanical medicines as comprehensive healthcare solutions for multifactorial diseases.
(© 2024. The Author(s), under exclusive licence to The Japanese Society of Hypertension.)
Databáze: MEDLINE
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