Analysis of the genus B othrops snake venom: An inter and intraspecific comparative study.
Autor: | Godoy TA; Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil.; Programa de Pós-Graduação Interunidades em Biotecnologia, São Paulo, Brazil.; Escola Superior do Instituto Butantan, São Paulo, Brazil., Lima EOV; Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil.; Programa de Pós-Graduação Interunidades em Biotecnologia, São Paulo, Brazil., Silveira GPM; Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil., Rodrigues FS; Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil., Sant'anna SS; Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil., Hatakeyama DM; Departamento de Bioquímica, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil., Grego KF; Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil., Tanaka-Azevedo AM; Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil.; Programa de Pós-Graduação Interunidades em Biotecnologia, São Paulo, Brazil.; Escola Superior do Instituto Butantan, São Paulo, Brazil. |
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Jazyk: | angličtina |
Zdroj: | Heliyon [Heliyon] 2024 Aug 30; Vol. 10 (17), pp. e37262. Date of Electronic Publication: 2024 Aug 30 (Print Publication: 2024). |
DOI: | 10.1016/j.heliyon.2024.e37262 |
Abstrakt: | The genus Bothrops are considered Category 1 of medical importance by the World Health Organization, responsible for approximately 85 % of snakebites occurring throughout Brazil. Main factors determining snake venom variations can be genetics, diet, gender, geographic distribution, age, or even seasonality. In this study, we compared the composition of protein profile, biochemical activities, and immunorecognition of toxins present in the venom of eight adults of Bothrops species ( B. alternatus, B. atrox, B. jararaca, B. jararacussu, B. leucurus, B. moojeni, B. neuwiedi and B. pauloensis ). The following methods were used to analyze the venoms: protein dosage; electrophoresis in polyacrylamide gel containing SDS; High Performance Liquid Chromatography - Reverse Phase; enzymatic activities, western blotting and Enzyme Linked Immuno Sorbent Assay. The results show inter and intraspecific differences in the electrophoretic profile. LAAO and PLA Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Thais Almeida de Godoy reports financial support was provided by Sao Paulo State Department for Health Coordination of Science Technology and Strategic Health Supplies. Anita Mitico Tanaka-Azevedo reports was provided by State of Sao Paulo Research Foundation. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.) |
Databáze: | MEDLINE |
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