Complex Biophysical and Computational Analyses of G-Quadruplex Ligands: The Porphyrin Stacks Back.

Autor: Satta G; Universita degli Studi di Sassari, Department of Chemical, Physical, Mathematical and Natural Sciences, ITALY., Trajkovski M; National Institute of Chemistry Slovenia, Slovenian NMR Centre, SLOVENIA., Cantara A; Institute of Biophysics Czech Academy of Sciences, Institute of Biophysics, CZECHIA., Mura M; Università degli Studi di Cagliari, Department of Chemistry and Geological Science, ITALY., Meloni C; Università degli Studi di Cagliari, Department of Chemistry and Geological Science, ITALY., Olla G; Università degli Studi di Cagliari, Department of Chemistry and Geological Science, ITALY., Dobrovolná M; Institute of Biophysics Czech Academy of Sciences, Institute of Biophysics, CZECHIA., Pisano L; Università degli Studi di Sassari, Department of Chemical, Physical, Mathematical and Natural Sciences, ITALY., Gaspa S; Università degli Studi di Sassari, Department of Chemical, Physical, Mathematical and Natural Sciences, ITALY., Salis A; Università degli Studi di Cagliari, Department of Chemistry and Geological Science, ITALY., De Luca L; Università degli Studi di Sassari, Department of Chemical, Physical, Mathematical and Natural Sciences, ITALY., Mocci F; Università degli Studi di Cagliari, Department of Chemistry and Geological Science, ITALY., Brazda V; Institute of Biophysics Czech Academy of Sciences, Institute of Biophysics, CZECHIA., Plavec J; National Institute of Chemistry Slovenia, Slovenian NMR Centre, SLOVENIA., Carraro M; University of Sassari, Department of Chemical, Physical, Mathematical and Natural Sciences, via Vienna 2, 07100, Sassari, ITALY.
Jazyk: angličtina
Zdroj: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2024 Sep 18, pp. e202402600. Date of Electronic Publication: 2024 Sep 18.
DOI: 10.1002/chem.202402600
Abstrakt: G-quadruplexes (G4s), as non-canonical DNA structures, attract a great deal of research interest in the molecular biology as well as in the material science fields. The use of small molecules as ligands for G-quadruplexes has emerged as a tool to regulate gene expression and telomeres maintenance. Meso-tetrakis-(N-methyl-4-pyridyl)porphyrin (TMPyP4) was shown as one of the first ligands for G-quadruplexes and it is still widely used. We report an investigation comprising molecular docking and dynamics, synthesis and multiple spectroscopic and spectrometric determinations on simple cationic porphyrins and their interaction with different DNA sequences. The study allowed to synthesize a few compounds that have shown to interact with DNA; the detailed characterization has shown that the presence of amide groups at the periphery improves selectivity for parallel G4s binding over other structures. Taking into account the ease of synthesis, 5,10,15,20-tetrakis-(1-acetamido-4-pyridyl)porphyrin bromide could be considered a better alternative to TMPyP4 in studies involving G4 binding.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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