Endothelin-1-Induced Persistent Ischemia in a Chicken Embryo Model.

Autor:	Kumari N; Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era's Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India., Prakash R; Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era's Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India., Siddiqui AJ; Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era's Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India., Waseem A; Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era's Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India., Khan MA; Era's Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India., Raza SS; Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era's Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India.; Department of Stem Cell Biology and Regenerative Medicine, Era's Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India.
Jazyk:	angličtina
Zdroj:	Bio-protocol [Bio Protoc] 2024 Sep 05; Vol. 14 (17), pp. e5060. Date of Electronic Publication: 2024 Sep 05 (Print Publication: 2024).
DOI:	10.21769/BioProtoc.5061
Abstrakt:	Current ischemic models strive to replicate ischemia-mediated injury. However, they face challenges such as inadequate reproducibility, difficulties in translating rodent findings to humans, and ethical, financial, and practical constraints that limit the accuracy of extensive research. This study introduces a novel approach to inducing persistent ischemia in 3-day-old chicken embryos using endothelin-1. The protocol targets the right vitelline arteries, validated with Doppler blood flow imaging and molecular biology experiments. This innovative approach facilitates the exploration of oxidative stress, inflammatory responses, cellular death, and potential drug screening suitability utilizing a 3-day-old chicken embryo. Key features • This model enables the evaluation and investigation of the pathology related to persistent ischemia • This model allows for the assessment of parameters like oxidative stress, inflammation, and cellular death • This model enables quantification of molecular changes at the nucleic acid and protein levels • This model allows for the efficient screening of drugs and their targets Graphical overview. Competing Interests: Competing interestsThe authors declare no competing interest. (©Copyright : © 2024 The Authors; This is an open access article under the CC BY-NC license.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu