Increased circulating LOX-1 + neutrophils activate T cells and demonstrate a pro-inflammatory role in allergic rhinitis.

Autor:	Deng XH; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China.; Department of Allergy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China., Huang LX; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China.; Department of Allergy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China., Sun Q; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China.; Department of Allergy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China., Li CG; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China.; Department of Allergy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China., Xie YC; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China.; Department of Allergy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China., Liu XQ; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China.; Department of Allergy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China., Fu QL; Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China.; Department of Allergy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, PR China.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Aug 16; Vol. 10 (17), pp. e36218. Date of Electronic Publication: 2024 Aug 16 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e36218
Abstrakt:	Background: Low-density neutrophils are heterogeneous immune cells with immunosuppressive (such as polymorphonuclear myeloid-derived suppressor cells [PMN-MDSC]) or pro-inflammatory (such as low-density granulocytes [LDG]) properties that have been well described in multiple cancers and immune diseases. However, its role in allergic rhinitis (AR) is still unclear. Methods: In the present study, we defined low-density neutrophils as CD14 - CD11B + CD15 + LOX-1 + (LOX-1 + neutrophils), and their levels in the peripheral blood (PB) were evaluated and compared between patients with AR and healthy donors using flow cytometric analysis. LOX-1 expression on polymorphonuclear neutrophils was identified. Carboxyfluorescein succinimidyl ester (CFSE)-stained CD3 + T cells were cultured alone or with LOX-1 + neutrophils, T cell proliferation was assessed using flow cytometry, and pro-inflammatory cytokines in the supernatants were detected using enzyme-linked immunosorbent assay (ELISA). Clinicopathological analyses were performed to gain a thorough understanding of LOX-1 + neutrophils. Results: We determined that LOX-1 + neutrophils were significantly increased in the PB of patients with AR, and LOX-1 expression in neutrophils from patients with AR was elevated. Interestingly, LOX-1 + neutrophils derived from patients with AR, unlike PMN-MDSC, promoted T cell proliferation and pro-inflammatory cytokine production. Moreover, clinicopathological analysis revealed that there was no any relation between circulating LOX-1 + neutrophil levels and the levels of IgE, age and sex. Conclusion: These findings indicate that elevated circulating LOX-1 + neutrophils play a pro-inflammatory role in AR. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu