Characterization of Composite Agarose-Collagen Hydrogels for Chondrocyte Culture.

Autor: Zigan C; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA., Benito Alston C; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA., Chatterjee A; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.; Department of Mechanical Engineering, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, Hyderabad, Telangana, India., Solorio L; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA., Chan DD; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA. chand@purdue.edu.; School of Mechanical Engineering, Purdue University, West Lafayette, IN, USA. chand@purdue.edu.
Jazyk: angličtina
Zdroj: Annals of biomedical engineering [Ann Biomed Eng] 2024 Sep 14. Date of Electronic Publication: 2024 Sep 14.
DOI: 10.1007/s10439-024-03613-x
Abstrakt: To elucidate the mechanisms of cellular mechanotransduction, it is necessary to employ biomaterials that effectively merge biofunctionality with appropriate mechanical characteristics. Agarose and collagen separately are common biopolymers used in cartilage mechanobiology and mechanotransduction studies but lack features that make them ideal for functional engineered cartilage. In this study, agarose is blended with collagen type I to create hydrogels with final concentrations of 4% w/v or 2% w/v agarose with 2 mg/mL collagen. We hypothesized that the addition of collagen into a high-concentration agarose hydrogel does not diminish mechanical properties. Acellular and cell-laden studies were completed to assess rheologic and compressive properties, contraction, and structural homogeneity in addition to cell proliferation and sulfated glycosaminoglycan production. Over 21 days in culture, cellular 4% agarose-2 mg/mL collagen I hydrogels seeded with primary murine chondrocytes displayed structural and bulk mechanical behaviors that did not significantly alter from 4% agarose-only hydrogels, cell proliferation, and continual glycosaminoglycan production, indicating promise toward the development of an effective hydrogel for chondrocyte mechanotransduction and mechanobiology studies.
(© 2024. The Author(s).)
Databáze: MEDLINE
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