Untargeted Metabolomics Analysis of Lactic Acid Bacteria Fermented Acanthopanax senticosus with Regard to Regulated Gut Microbiota in Mice.

Autor: Su Y; Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China., Fu X; College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng 252000, China., Zhuang P; Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2024 Aug 28; Vol. 29 (17). Date of Electronic Publication: 2024 Aug 28.
DOI: 10.3390/molecules29174074
Abstrakt: Previous studies have shown that Acanthopanax senticosus (AS) has a beneficial preventive and therapeutic effect on colitis. The fermentation of lactic acid bacteria (LAB) can alter the efficacy of AS by modifying or producing new compounds with potential bioactive properties. However, the specific components and mechanisms that enhance the efficacy are still unclear. In the present experiment, untargeted metabolomics was used to analyze the changes in active components before and after LAB fermentation of AS. The aim was to explain the mechanism of AS fermentation in treating colitis using a colitis model in mice. The results indicated that the fermentation of LAB could enhance the levels of total flavonoids and total polyphenols in FAS. Additionally, the beneficial components such as Delphinidin chloride, Diosmetin, Psoralidin, and Catechol significantly increased ( p < 0.05). The colitis treatment experiment demonstrated that fermented AS could alleviate symptoms and improve the morphology of colitis in mice by enhancing antioxidant enzymes like CAT, T-SOD, and T-AOC. It also regulated the composition and abundance of intestinal flora species, such as Lactobacillus and Pseudogracilibacillus . The effectiveness of fermented AS was significantly superior to that of unfermented AS ( p < 0.05). In conclusion, this study contributes to the application of lactic acid bacteria in AS fermentation and reveals the mechanism of fermentation AS for colitis.
Databáze: MEDLINE
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