| Autor: |
Rossi JO; Occupational Medicine, Marilia School of Medicine (FAMEMA), Marilia 17519-030, Brazil., Araujo EMC; Department of Biological Sciences, Bauru School of Dentistry (FOB/USP), University of São Paulo, Bauru 17012-901, Brazil., Camargo MEC; Medical School, University of Marilia (UNIMAR), Marilia 17525-902, Brazil., Ferreira Junior RS; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (University Estadual Paulista, UNESP), Botucatu 18610-307, Brazil.; Graduate Programs in Tropical Diseases and Clinical Research, Botucatu Medical School (FMB), São Paulo State University (UNESP-University Estadual Paulista), Botucatu 18618-687, Brazil., Barraviera B; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (University Estadual Paulista, UNESP), Botucatu 18610-307, Brazil.; Graduate Programs in Tropical Diseases and Clinical Research, Botucatu Medical School (FMB), São Paulo State University (UNESP-University Estadual Paulista), Botucatu 18618-687, Brazil., Miglino MA; Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marilia 17525-902, Brazil.; Postgraduate Program in Animal Health, Production and Environment, University of Marilia (UNIMAR), Marilia 17525-902, Brazil., Nogueira DMB; Department of Anatomy, Faculty of Higher Education of the Interior of São Paulo (FAIP), Marilia 17512-130, Brazil., Reis CHB; Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marilia 17525-902, Brazil., Gil GE; Medical School, University of Marilia (UNIMAR), Marilia 17525-902, Brazil., Vinholo TR; Medical School, University of Marilia (UNIMAR), Marilia 17525-902, Brazil., Soares TP; Medical School, University of Marilia (UNIMAR), Marilia 17525-902, Brazil., Buchaim RL; Department of Biological Sciences, Bauru School of Dentistry (FOB/USP), University of São Paulo, Bauru 17012-901, Brazil.; Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), Sao Paulo 05508-270, Brazil., Buchaim DV; Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marilia 17525-902, Brazil.; Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), Sao Paulo 05508-270, Brazil.; Medical School, University Center of Adamantina (UNIFAI), Adamantina 17800-000, Brazil. |
| Abstrakt: |
Biomaterials and biopharmaceuticals for correcting large bone defects are a potential area of translational science. A new bioproduct, purified from snake venom and fibrinogen from buffalo blood, aroused interest in the repair of venous ulcers. Expanding potential uses, it has also been used to form biocomplexes in combination with bone grafts, associated with physical therapies or used alone. The aim of this preclinical study was to evaluate low-level laser photobiomodulation (PBM) in critical defects in the calvaria of rats filled with nanohydroxyapatite (NH) associated with the heterologous fibrin biopolymer (HFB). Sixty animals were used, divided into six groups ( n = 10 each): G1 (NH); G2 (HFB); G3 (NH + HFB); G4 (NH + PBM); G5 (HFB + PBM); G6 (NH + HFB + PBM). PBM simultaneously used red (R) and infrared (IR) light emission, applied intraoperatively and twice a week, until the end of the experiment at 42 days. Microtomography, bone formation can be seen initially at the margins of the defect, more evident in G5. Microscopically, bone formation demonstrated immature and disorganized trabeculation at 14 days, with remnants of grafting materials. At 42 days, the percentage of new bone formed was higher in all groups, especially in G5 (HFB, 45.4 ± 3.82), with collagen fibers at a higher degree of maturation and yellowish-green color in the birefringence analysis with Picrosirius-red. Therefore, it is concluded that the HFB + PBM combination showed greater effectiveness in the repair process and presents potential for future clinical studies. |
