Investigation of the potential teratogenic effects of fructose on the embryo using the rat whole embryo culture model.

Autor: Gür HE; Department of Histology & Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey. Electronic address: hemelgur@gmail.com., Balcıoğlu E; Department of Histology & Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey., Patat D; Department of Anatomy, Faculty of Medicine, Medipol University, Ankara, Turkey., Uçar S; Department of Anatomy, Faculty of Medicine, Erciyes University, Kayseri, Turkey., Gür FM; Department of Histology & Embryology, Faculty of Medicine, Niğde Ömer Halisdemir University, Turkey., Yalçın B; Department of Histology & Embryology, Faculty of Medicine, Adiyaman University, Turkey., Nisari M; Department of Anatomy, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2024 Nov; Vol. 193, pp. 114985. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1016/j.fct.2024.114985
Abstrakt: Excessive consumption of fructose-sweetened foods and beverages is a growing concern worldwide. Studies have demonstrated that fructose consumption before and during pregnancy can result in adverse outcomes such as decreased decidualization, increased fetal losses, and low birth weight. The study investigated the teratogenic effects of fructose on rat embryos during organogenesis using whole embryo culture. Within the scope of the study, 4 groups were formed as control, low, medium, and high-dose fructose (HDF) with 10 embryos in each group. The 9.5-day-old rat embryos were cultured with different concentrations of fructose (1, 5 and 10 mM) for 48 h and the possible effects of fructose were examined using morphological scoring, histochemistry, immunofluorescence, and TUNEL methods. According to the analyses, protein synthesis and proliferation were decreased, vascular formation was suppressed, and apoptosis was increased in embryos exposed to fructose, especially at concentrations of 5 mM and above. According to the morphological scoring results, it was determined that heart, hind limb, and somite development were retarded in all experimental groups compared to the control group, developmental retardation increased in direct proportion to fructose concentration, and also significant malformations were observed in all parameters examined in the HDF group. In addition, analysis of yolk sac diameter, head length, crown rump length and somite numbers showed that these parameters were significantly decreased in all experimental groups. End of the study, it was concluded that fructose at concentrations of 1 mM and above may induce embryonic development retardation and other anomalies by decreasing protein synthesis and cell proliferation, suppressing vascular formation, and increasing apoptosis in embryonic tissues.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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