The use of metagenomic and untargeted metabolomics in the analysis of the effects of the Lycium barbarum glycopeptide on allergic airway inflammation induced by Artemesia annua pollen.

Autor: Bai M; School of Basic Medicine, Ningxia Medical University, Yinchuan, China; Department of Rheumatology and Immunology, Peking Union Medical College Hospital, Beijing, China., Zhou Z; School of Inspection, Ningxia Medical University, Yinchuan, China; Research Center for Medical Science and Technology, Ningxia Medical University, Yinchuan, China., Yin M; Department of Respiratory and Critical Care Medicine, Hospital of Cardiovascular and Cerebrovascular Diseases, General Hospital of Ningxia Medical University, Yinchuan, China., Wang M; Ningxia Institute of Medical Science, Yinchuan, China; Research Center for Medical Science and Technology, Ningxia Medical University, Yinchuan, China. Electronic address: 398604011@qq.com., Gao X; Department of Otolaryngology Head and Neck Surgery, General Hospital of Ningxia Medical University, Yinchuan, China. Electronic address: monsieurgao@163.com., Zhao J; School of Basic Medicine, Ningxia Medical University, Yinchuan, China; Ningxia Institute of Medical Science, Yinchuan, China; Research Center for Medical Science and Technology, Ningxia Medical University, Yinchuan, China. Electronic address: zhaojq@nxmu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2025 Jan 30; Vol. 337 (Pt 1), pp. 118816. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1016/j.jep.2024.118816
Abstrakt: Ethnopharmacological Relevance: The prevalence of allergic airway inflammation (AAI) worldwide is high. Artemisia annua L. pollen is spread worldwide, and allergic diseases caused by its plant polysaccharides, which are closely related to the intestinal microbiota, have anti-inflammatory effects. Further isolation and purification of Lycium barbarum L. yielded its most effective component Lycium barbarum L. glycopeptide (LbGP), which can inhibit inflammation in animal models. However, its therapeutic effect on AAI and its mechanism of regulating the intestinal flora have not been fully investigated.
Aim of the Study: To explore LbGP in APE-induced immunological mechanisms of AAI and the interaction mechanism of the intestinal flora and metabolites.
Methods: A mouse model of AAI generated from Artemisia annua pollen was constructed, and immunological indices related to the disease were examined. A combination of macrogenomic and metabolomic analyses was used to investigate the effects of LbGP on the gut microbial and metabolite profiles of mice with airway inflammation.
Results: LbGP effectively alleviated Artemisia. annua pollen extract (APE)-induced AAI, corrected Th1/Th2 immune dysregulation, decreased Th17 cells, increased Treg cells, and altered the composition and function of the intestinal microbiota. LbGP treatment increased the number of OdoribacterandDuncaniella in the intestines of the mice, but the numble of Alistipes and Ruminococcus decreased. Metabolite pathway enrichment analysis were used to determine the effects of taurine and hypotaurine metabolism, bile acid secretion, and pyrimidine metabolism pathways on disease.
Conclusion: Our results revealed significant changes in the macrogenome and metabolome following APE and LbGP intervention, revealed potential correlations between gut microbial species and metabolites, and highlighted the beneficial effects of LbGP on AAI through the modulation of the gut microbiome and host metabolism.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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